AKAP6 Orchestrates the Nuclear Envelope Microtubule-organizing Center by Linking Golgi and Nucleus Via AKAP9

Overview

Journal Elife

Specialty Biology

Date 2020 Dec 9

PMID 33295871

Citations 21

Authors

Silvia Vergarajauregui

Robert Becker

Ulrike Steffen

Maria Sharkova

Tilman Esser

Jana Petzold

Florian Billing

Michael S Kapiloff

George Schett

Ingo Thievessen

Felix B Engel

Affiliations

Soon will be listed here.

Abstract

The switch from centrosomal microtubule-organizing centers (MTOCs) to non-centrosomal MTOCs during differentiation is poorly understood. Here, we identify AKAP6 as key component of the nuclear envelope MTOC. In rat cardiomyocytes, AKAP6 anchors centrosomal proteins to the nuclear envelope through its spectrin repeats, acting as an adaptor between nesprin-1α and Pcnt or AKAP9. In addition, AKAP6 and AKAP9 form a protein platform tethering the Golgi to the nucleus. Both Golgi and nuclear envelope exhibit MTOC activity utilizing either AKAP9, or Pcnt-AKAP9, respectively. AKAP6 is also required for formation and activity of the nuclear envelope MTOC in human osteoclasts. Moreover, ectopic expression of AKAP6 in epithelial cells is sufficient to recruit endogenous centrosomal proteins. Finally, AKAP6 is required for cardiomyocyte hypertrophy and osteoclast bone resorption activity. Collectively, we decipher the MTOC at the nuclear envelope as a bi-layered structure generating two pools of microtubules with AKAP6 as a key organizer.

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