Saturation Transfer Properties of Tumour Xenografts Derived from Prostate Cancer Cell Lines 22Rv1 and DU145

Overview

Journal Sci Rep

Specialty Science

Date 2020 Dec 5

PMID 33277574

Citations 1

Authors

Ziyu Tan

Wilfred W Lam

Wendy Oakden

Leedan Murray

Margaret M Koletar

Stanley K Liu

Greg J Stanisz

Affiliations

Soon will be listed here.

Abstract

Histopathology is currently the most reliable tool in assessing the aggressiveness and prognosis of solid tumours. However, developing non-invasive modalities for tumour evaluation remains crucial due to the side effects and complications caused by biopsy procedures. In this study, saturation transfer MRI was used to investigate the microstructural and metabolic properties of tumour xenografts in mice derived from the prostate cancer cell lines 22Rv1 and DU145, which express different aggressiveness. The magnetization transfer (MT) and chemical exchange saturation transfer (CEST) effects, which are associated with the microstructural and metabolic properties in biological tissue, respectively, were analyzed quantitatively and compared amongst different tumour types and regions. Histopathological staining was performed as a reference. Higher cellular density and metabolism expressed in more aggressive tumours (22Rv1) were associated with larger MT and CEST effects. High collagen content in the necrotic regions might explain their higher MT effects compared to tumour regions.

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