A Lipid Bilayer Formed on a Hydrogel Bead for Single Ion Channel Recordings

Overview

Journal Micromachines (Basel)

Publisher MDPI

Date 2020 Dec 4

PMID 33271761

Citations 2

Authors

Minako Hirano

Daiki Yamamoto

Mami Asakura

Tohru Hayakawa

Shintaro Mise

Akinobu Matsumoto

Toru Ide

Affiliations

Soon will be listed here.

Abstract

Ion channel proteins play important roles in various cell functions, making them attractive drug targets. Artificial lipid bilayer recording is a technique used to measure the ion transport activities of channel proteins with high sensitivity and accuracy. However, the measurement efficiency is low. In order to improve the efficiency, we developed a method that allows us to form bilayers on a hydrogel bead and record channel currents promptly. We tested our system by measuring the activities of various types of channels, including gramicidin, alamethicin, α-hemolysin, a voltage-dependent anion channel 1 (VDAC1), a voltage- and calcium-activated large conductance potassium channel (BK channel), and a potassium channel from (KcsA channel). We confirmed the ability for enhanced measurement efficiency and measurement system miniaturizion.

Citing Articles

Efficient single-channel current measurements of the human BK channel using a liposome-immobilized gold probe.

Hirano M, Asakura M, Ide T Anal Sci. 2024; .

PMID: 39707141 DOI: 10.1007/s44211-024-00707-3.

Characterizing the Structure and Interactions of Model Lipid Membranes Using Electrophysiology.

El-Beyrouthy J, Freeman E Membranes (Basel). 2021; 11(5).

PMID: 33925756 PMC: 8145864. DOI: 10.3390/membranes11050319.

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