New Insights into the Microbial Profiles of Infected Root Canals in Traumatized Teeth

Overview

Journal J Clin Med

Specialty General Medicine

Date 2020 Dec 2

PMID 33260621

Citations 9

Authors

Lokeshwaran Manoharan

Malin Brundin

Olena Rakhimova

Luis Chavez de Paz

Nelly Romani Vestman

Affiliations

Soon will be listed here.

Abstract

Traumatic dental injuries in young individuals are often exposed to the invasion of oral microorganisms that leads to pulp necrosis. Infective necrosis in permanent teeth not-fully-developed causes aberrant root formation. Regeneration endodontic treatments (RETs) have shown promising results by promoting continued root development by stem cells. Critical to the success of RET is the thorough disinfection of the pulpal space. To establish effective antimicrobial protocols for root canal disinfection, the invading microorganisms need to be identified. In the present study, we use a combination of culture-based and high-throughput molecular sequencing techniques to investigate the microbial profiles from traumatized teeth (30 cases) and controls, i.e., teeth with pulp infections not caused by trauma (32 cases). Overall, a high microbial diversity in traumatized necrotic teeth was observed. Eubacterium yurii subsps. yurii and margaretiae, as well as key 'bridging oral species' F. nucleatum sp., Polymorphum and Corynebacterium matruchotti, were highly associated with traumatized teeth. The microbial compositions of traumatized teeth differed considerably from those of infected teeth not caused by trauma. Age and tooth position also influence microbial compositions. In conclusion, we show that the root canal microflora of traumatized teeth is highly diverse, and it differs from root canal infections not caused by trauma.

Citing Articles

Microbiological assessment of success and failure in pulp revitalization: a randomized clinical trial using calcium hydroxide and chlorhexidine gluconate in traumatized immature necrotic teeth.

Wikstrom A, Romani Vestman N, Rakhimova O, Lazaro Gimeno D, Tsilingaridis G, Brundin M J Oral Microbiol. 2024; 16(1):2343518.

PMID: 38665416 PMC: 11044761. DOI: 10.1080/20002297.2024.2343518.

Exploring the impact of oral bacteria remnants on stem cells from the Apical papilla: mineralization potential and inflammatory response.

Zymovets V, Rakhimova O, Wadelius P, Schmidt A, Brundin M, Kelk P Front Cell Infect Microbiol. 2023; 13:1257433.

PMID: 38089810 PMC: 10711090. DOI: 10.3389/fcimb.2023.1257433.

Microbial identification from traumatized immature permanent teeth with periapical lesions using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

Sharaf P, El Backly R, Sherif R, Zaazou A, Hafez S BMC Oral Health. 2022; 22(1):661.

PMID: 36587207 PMC: 9805193. DOI: 10.1186/s12903-022-02562-y.

Transcriptome Analysis Reveals Modulation of Human Stem Cells from the Apical Papilla by Species Associated with Dental Root Canal Infection.

Razghonova Y, Zymovets V, Wadelius P, Rakhimova O, Manoharan L, Brundin M Int J Mol Sci. 2022; 23(22).

PMID: 36430898 PMC: 9695896. DOI: 10.3390/ijms232214420.

Taxonomic abundance in primary and secondary root canal infections.

Ordinola-Zapata R, Costalonga M, Nixdorf D, Dietz M, Schuweiler D, Lima B Int Endod J. 2022; 56(2):278-288.

PMID: 36334085 PMC: 10100057. DOI: 10.1111/iej.13864.

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