Stathmin! An Immunohistochemical Analysis of the Novel Marker in Oral Squamous Cell Carcinoma and Oral Leukoplakia

Overview

Journal Asian Pac J Cancer Prev

Specialty Oncology

Date 2020 Nov 28

PMID 33247690

Citations 2

Authors

Purnima Vadla

Sivaranjani Yeluri

G Deepthi

Venkateswara Rao Guttikonda

Sravya Taneeru

Srikanth Naramala

Affiliations

Soon will be listed here.

Abstract

Background: Stathmin is an intracellular phosphoprotein that controls the microtubule dynamics by further regulating proper attachment and alignment of chromosomes in a dividing cell. Thus, any mutation or aberrantly expressed protein that reduces the fidelity of spindle assembly will enhance chromosomal instability contributing to aneuploidy. Oral Squamous Cell Carcinoma is an extensively studied malignancy that occurs due to accumulated genetic changes due to carcinogens. The current study is done to evaluate the stathmin role and its expression in OSCC and Oral epithelial dysplasia (OED).

Objective: The aim of the present study is to evaluate the role of stathmin in OSCC and Oral dysplasia and also to correlate the expression of Stathmin with respect to the different histopathological grades of OED and OSCC.

Materials And Methods: 30 neutral buffered formalin fixed, paraffin embedded (FFPE) tissues of Oral Leukoplakia/OED and 30 FFPE tissues of OSCC were subjected to immunohistochemistry with stathmin antibody. Five fields of each case with 300 cells were examined and a mean percentage of positive-stained slides were determined. The percentages were recorded accordingly with their respective histological grades. The results were analysed statistically.

Results: The results of the present study demonstrated higher mean values of stathmin in tissues with OSCC (2.50) compared to leukoplakia (2.11) and normal tissues (0.00) with a high level of statistical significance (0.0001). There is also an increase in the percentage levels of stathmin with increase in the histological grade of differentiation in OSCC as well as leukoplakia.

Conclusion: The present study found a statistical correlation between increased grades of the disease with expression levels of stathmin. This confirms that stathmin expression can contribute to disease progression and that stathmin might have a potential role as an early diagnostic biomarker and can be a therapeutic target for OSCC. <br />.

Citing Articles

Expression of stathmin in oral squamous cell carcinoma and its correlation with tumour proliferation.

Rana S, Mondal P, Mandal M, Datta P, Maji I, Chakraborty J J Oral Maxillofac Pathol. 2023; 27(1):103-108.

PMID: 37234316 PMC: 10207213. DOI: 10.4103/jomfp.jomfp_202_22.

Molecular Biomarkers of Malignant Transformation in Head and Neck Dysplasia.

Ranganath K, Feng A, Franco R, Varvares M, Faquin W, Naunheim M Cancers (Basel). 2022; 14(22).

PMID: 36428690 PMC: 9688631. DOI: 10.3390/cancers14225581.

References

Hsu H, Li C, Lee S, Wu W, Chen T, Chang K . Overexpression of stathmin 1 confers an independent prognostic indicator in nasopharyngeal carcinoma. Tumour Biol. 2013; 35(3):2619-29. DOI: 10.1007/s13277-013-1345-3. View

Friedrich B, Gronberg H, Landstrom M, Gullberg M, Bergh A . Differentiation-stage specific expression of oncoprotein 18 in human and rat prostatic adenocarcinoma. Prostate. 1995; 27(2):102-9. DOI: 10.1002/pros.2990270207. View

Belmont L, Mitchison T . Identification of a protein that interacts with tubulin dimers and increases the catastrophe rate of microtubules. Cell. 1996; 84(4):623-31. DOI: 10.1016/s0092-8674(00)81037-5. View

Choi S, Myers J . Molecular pathogenesis of oral squamous cell carcinoma: implications for therapy. J Dent Res. 2007; 87(1):14-32. DOI: 10.1177/154405910808700104. View

Hanash S, Strahler J, Kuick R, Chu E, Nichols D . Identification of a polypeptide associated with the malignant phenotype in acute leukemia. J Biol Chem. 1988; 263(26):12813-5. View

Warnakulasuriya S, Ariyawardana A . Malignant transformation of oral leukoplakia: a systematic review of observational studies. J Oral Pathol Med. 2015; 45(3):155-66. DOI: 10.1111/jop.12339. View

Curmi P, Nogues C, Lachkar S, Carelle N, Gonthier M, Sobel A . Overexpression of stathmin in breast carcinomas points out to highly proliferative tumours. Br J Cancer. 2000; 82(1):142-50. PMC: 2363189. DOI: 10.1054/bjoc.1999.0891. View

Tian X, Tian Y, Sarich N, Wu T, Birukova A . Novel role of stathmin in microtubule-dependent control of endothelial permeability. FASEB J. 2012; 26(9):3862-74. PMC: 3425818. DOI: 10.1096/fj.12-207746. View

Fedchenko N, Reifenrath J . Different approaches for interpretation and reporting of immunohistochemistry analysis results in the bone tissue - a review. Diagn Pathol. 2014; 9:221. PMC: 4260254. DOI: 10.1186/s13000-014-0221-9. View

10.

Akhtar J, Wang Z, Zhang Z, Bi M . Lentiviral-mediated RNA interference targeting stathmin1 gene in human gastric cancer cells inhibits proliferation in vitro and tumor growth in vivo. J Transl Med. 2013; 11:212. PMC: 3848762. DOI: 10.1186/1479-5876-11-212. View

11.

Rubin C, Atweh G . The role of stathmin in the regulation of the cell cycle. J Cell Biochem. 2004; 93(2):242-50. DOI: 10.1002/jcb.20187. View

12.

Nakashima D, Uzawa K, Kasamatsu A, Koike H, Endo Y, Saito K . Protein expression profiling identifies maspin and stathmin as potential biomarkers of adenoid cystic carcinoma of the salivary glands. Int J Cancer. 2005; 118(3):704-13. DOI: 10.1002/ijc.21318. View

13.

Liu F, Sun Y, Xu Y, Liu F, Wang L, Zhao X . Expression and phosphorylation of stathmin correlate with cell migration in esophageal squamous cell carcinoma. Oncol Rep. 2012; 29(2):419-24. PMC: 3583596. DOI: 10.3892/or.2012.2157. View

14.

Marafioti T, Copie-Bergman C, Calaminici M, Paterson J, Shende V, Liu H . Another look at follicular lymphoma: immunophenotypic and molecular analyses identify distinct follicular lymphoma subgroups. Histopathology. 2013; 62(6):860-75. DOI: 10.1111/his.12076. View

15.

Wei S, Lin F, Wang X, Gao P, Zhang H . Prognostic significance of stathmin expression in correlation with metastasis and clinicopathological characteristics in human ovarian carcinoma. Acta Histochem. 2007; 110(1):59-65. DOI: 10.1016/j.acthis.2007.06.002. View

16.

Alaiya A, Franzen B, Fujioka K, Moberger B, Schedvins K, Silfversvard C . Phenotypic analysis of ovarian carcinoma: polypeptide expression in benign, borderline and malignant tumors. Int J Cancer. 1997; 73(5):678-83. DOI: 10.1002/(sici)1097-0215(19971127)73:5<678::aid-ijc11>3.0.co;2-2. View

17.

Cheng A, Huang W, Chen Z, Peng F, Zhang P, Li M . Identification of novel nasopharyngeal carcinoma biomarkers by laser capture microdissection and proteomic analysis. Clin Cancer Res. 2008; 14(2):435-45. DOI: 10.1158/1078-0432.CCR-07-1215. View

18.

Biggins S, Walczak C . Captivating capture: how microtubules attach to kinetochores. Curr Biol. 2003; 13(11):R449-60. DOI: 10.1016/s0960-9822(03)00369-5. View

19.

Luo X, Mookerjee B, Ferrari A, Mistry S, Atweh G . Regulation of phosphoprotein p18 in leukemic cells. Cell cycle regulated phosphorylation by p34cdc2 kinase. J Biol Chem. 1994; 269(14):10312-8. View

20.

Lu Y, Liu C, Cheng H, Xu Y, Jiang J, Xu J . Stathmin, interacting with Nf-κB, promotes tumor growth and predicts poor prognosis of pancreatic cancer. Curr Mol Med. 2014; 14(3):328-39. DOI: 10.2174/1566524014666140228120913. View