Lateral Flow Immunoassay to Detect the Addition of Beef, Pork, Lamb, and Horse Muscles in Raw Meat Mixtures and Finished Meat Products

Overview

Journal Foods

Specialty Biotechnology

Date 2020 Nov 18

PMID 33203004

Citations 5

Authors

Elena A Zvereva

Demid S Popravko

Olga D Hendrickson

Natalia L Vostrikova

Irina M Chernukha

Boris B Dzantiev

Anatoly V Zherdev

Affiliations

Soon will be listed here.

Abstract

A lateral flow immunoassay for sensitive detection of skeletal troponin I (TnI) as a specific, thermostable marker of muscle tissue was developed. Due to the antibodies' choice, the assay specifically detects mammalian TnI (in beef, pork, lamb, and horse) but does not detect bird TnI (in chicken or turkey), thus enabling differentiation of these types of raw meat materials. The assay is based on a sandwich format of the analysis using gold nanoparticles as labels. The time of the assay is 15 min, and the TnI detection limit is 25 ng/mL. A buffer solution is proposed for efficient extraction of TnI from muscle tissues and from finished meat products that have undergone technological processing (smoking-cooking-smoking, cooking and smoking). The possibility of detecting beef addition in minced chicken down to 1% was demonstrated.

Citing Articles

Double lateral flow immunosensing of undeclared pork and chicken components of meat products.

Zvereva E, Hendrickson O, Dzantiev B, Zherdev A J Food Sci Technol. 2024; 61(6):1148-1156.

PMID: 38562594 PMC: 10981650. DOI: 10.1007/s13197-024-05944-y.

Towards halal pharmaceutical: Exploring alternatives to animal-based ingredients.

Herdiana Y, Sofian F, Shamsuddin S, Rusdiana T Heliyon. 2024; 10(1):e23624.

PMID: 38187251 PMC: 10770512. DOI: 10.1016/j.heliyon.2023.e23624.

Lateral Flow Test System to Control Total Content of Muscle Tissues in Raw Meat Products.

Zvereva E, Hendrickson O, Dzantiev B, Zherdev A Sensors (Basel). 2022; 22(24).

PMID: 36560100 PMC: 9783880. DOI: 10.3390/s22249724.

Rapid Full-Cycle Technique to Control Adulteration of Meat Products: Integration of Accelerated Sample Preparation, Recombinase Polymerase Amplification, and Test-Strip Detection.

Ivanov A, Popravko D, Safenkova I, Zvereva E, Dzantiev B, Zherdev A Molecules. 2021; 26(22).

PMID: 34833896 PMC: 8622786. DOI: 10.3390/molecules26226804.

Antibodies as Biosensors' Key Components: State-of-the-Art in Russia 2020-2021.

Rudenko N, Fursova K, Shepelyakovskaya A, Karatovskaya A, Brovko F Sensors (Basel). 2021; 21(22).

PMID: 34833687 PMC: 8624206. DOI: 10.3390/s21227614.

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