Method for Cryopreservation of Paramoeba Perurans

Overview

Journal J Fish Dis

Date 2020 Nov 7

PMID 33159703

Citations 3

Authors

Christiane Trosse

Mats Kindt

Steffen Blindheim

Linda Andersen

Are Nylund

Affiliations

Soon will be listed here.

Abstract

Paramoeba perurans causes amoebic gill disease (AGD), which is a major problem in aquaculture worldwide. The parasite can be cultured in vitro, but to this date, no method for long-term storage of the clones exists. In this study, we describe a method for cryopreservation of Paramoeba perurans. The method was successfully employed on four out the five clones we tested. The thawing success rate, that is the percentage of successfully thawed vials relative to the total number of vials that were thawed, differed for the clones and ranged from 25% to 100%. The age of the clones seemed to have a negative impact on the ability to survive cryopreservation.

Citing Articles

Growth characteristics and morphology of Paramoeba perurans from Atlantic salmon Salmo salar L. and ballan wrasse Labrus bergylta in Norway.

Blindheim S, Andersen L, Trosse C, Karlsbakk E, Nylund A Parasit Vectors. 2023; 16(1):112.

PMID: 36959596 PMC: 10037839. DOI: 10.1186/s13071-023-05715-2.

Experimental Challenge Models and In Vitro Models to Investigate Efficacy of Treatments and Vaccines against Amoebic Gill Disease.

Hudson J, Nowak B Microorganisms. 2021; 9(4).

PMID: 33808191 PMC: 8065880. DOI: 10.3390/microorganisms9040710.

Method for cryopreservation of Paramoeba perurans.

Trosse C, Kindt M, Blindheim S, Andersen L, Nylund A J Fish Dis. 2020; 44(6):739-745.

PMID: 33159703 PMC: 8246932. DOI: 10.1111/jfd.13295.

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