Rapid Detection of Influenza Virus in Cell Culture by Indirect Immunoperoxidase Staining with Type-specific Monoclonal Antibodies

Overview

Journal Diagn Microbiol Infect Dis

Specialties Infectious Diseases
Microbiology
Pathology

Date 1987 Aug 1

PMID 3315405

Citations 6

Authors

P D Swenson

M H Kaplan

Affiliations

Soon will be listed here.

Abstract

Seventy respiratory specimens culture-positive for influenza virus were stored at -70 degrees C and retested in duplicate by virus isolation and by indirect immunoperoxidase (IPA) staining of cell monolayers 24 hr postinoculation using influenza type-specific monoclonal antibodies. The IPA stain was positive for 24 of 28 specimens from which influenza virus was reisolated and for eight specimens from which influenza virus was not reisolated.

Citing Articles

Use of monoclonal antibodies for rapid diagnosis of respiratory viruses in a community hospital.

Irmen K, KELLEHER J Clin Diagn Lab Immunol. 2000; 7(3):396-403.

PMID: 10799452 PMC: 95885. DOI: 10.1128/CDLI.7.3.396-403.2000.

Application of subtype-specific monoclonal antibodies for rapid detection and identification of influenza A and B viruses.

Ueda M, Maeda A, Nakagawa N, Kase T, Kubota R, Takakura H J Clin Microbiol. 1998; 36(2):340-4.

PMID: 9466738 PMC: 104539. DOI: 10.1128/JCM.36.2.340-344.1998.

Physical and chemical methods for enhancing rapid detection of viruses and other agents.

Hughes J Clin Microbiol Rev. 1993; 6(2):150-75.

PMID: 8472247 PMC: 358275. DOI: 10.1128/CMR.6.2.150.

Rapid diagnosis of influenza A and B by 24-h fluorescent focus assays.

Stokes C, Bernstein J, Kyger S, Hayden F J Clin Microbiol. 1988; 26(7):1263-6.

PMID: 3045148 PMC: 266589. DOI: 10.1128/jcm.26.7.1263-1266.1988.

Detection of influenza virus by centrifugal inoculation of MDCK cells and staining with monoclonal antibodies.

Mills R, Cain K, Woods G J Clin Microbiol. 1989; 27(11):2505-8.

PMID: 2808674 PMC: 267067. DOI: 10.1128/jcm.27.11.2505-2508.1989.