Use of Monoclonal Antibodies for Rapid Diagnosis of Respiratory Viruses in a Community Hospital

Overview

Journal Clin Diagn Lab Immunol

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 2000 May 9

PMID 10799452

Citations 11

Authors

K E Irmen

J J KELLEHER

Affiliations

Soon will be listed here.

Abstract

An indirect fluorescence antibody (IFA) procedure was used for the rapid detection of respiratory viruses in direct clinical specimens and for determining the epidemiology of viruses in a community hospital setting. Viral respiratory diseases were monitored for 10 consecutive respiratory seasons. The Bartels Viral Respiratory Screening and Identification Kit is an IFA method that contains pooled and individual monoclonal antibodies for seven common respiratory viruses. Compared with 8,670 conventional tube cell cultures, IFA staining of direct patient specimens had an overall sensitivity of 84.2% and a specificity of 87.7%. Yearly epidemics of respiratory syncytial virus were seen with alternating short and long intervals between successive periods when virus was isolated. Epidemics following short intervals were more severe. Influenza A virus epidemics occurred yearly, and influenza B virus activity was seen generally every other year. When influenza A and influenza B viruses were cocirculating in a given season, the months of peak activity of one virus were always within 1 month of the peak activity of the other virus. Parainfluenza virus type 1 was detected in the autumn of odd-numbered years, and parainfluenza type 2 virus was seen usually in the autumn of even-numbered years. Parainfluenza type 3 virus and adenovirus were the most ubiquitous agents, with peak incidence occurring in the late winter to spring.

Citing Articles

Viral burden and diversity in acute respiratory tract infections in hospitalized children in wet and dry zones of Sri Lanka.

Jayaweera J, Morel A, Abeykoon A, Pitchai F, Kothalawela H, Peiris J PLoS One. 2021; 16(12):e0259443.

PMID: 34919553 PMC: 8682885. DOI: 10.1371/journal.pone.0259443.

Discovery and characterization of high-affinity, potent SARS-CoV-2 neutralizing antibodies via single B cell screening.

Schardt J, Pornnoppadol G, Desai A, Park K, Zupancic J, Makowski E Sci Rep. 2021; 11(1):20738.

PMID: 34671080 PMC: 8528929. DOI: 10.1038/s41598-021-99401-x.

Influenza-associated paediatric respiratory hospitalizations in China, 1996-2012: a systematic analysis.

Shang M, Lafond K, McFarland J, Zhou S, Klena J, Widdowson M Western Pac Surveill Response J. 2019; 9(5 Suppl 1):35-43.

PMID: 31832252 PMC: 6902651. DOI: 10.5365/wpsar.2018.9.1.004.

Diversity of respiratory viruses detected among hospitalized children with acute lower respiratory tract infections at Hospital Serdang, Malaysia.

Etemadi M, Jalilian F, Othman N, Lye M, Ansari S, Yubbu P J Virol Methods. 2019; 269:1-6.

PMID: 30910688 PMC: 7172173. DOI: 10.1016/j.jviromet.2019.03.013.

Improving Accuracy of Influenza-Associated Hospitalization Rate Estimates.

Millman A, Reed C, Kirley P, Aragon D, Meek J, Farley M Emerg Infect Dis. 2015; 21(9):1595-601.

PMID: 26292017 PMC: 4550134. DOI: 10.3201/eid2109.141665.

References

Gardner P, MCQUILLIN J, McGuckin R . The late detection of respiratory syncytial virus in cells of respiratory tract by immunofluorescence. J Hyg (Lond). 1970; 68(4):575-80. PMC: 2130874. DOI: 10.1017/s0022172400042509. View

Denny F, CLYDE Jr W . Acute lower respiratory tract infections in nonhospitalized children. J Pediatr. 1986; 108(5 Pt 1):635-46. DOI: 10.1016/s0022-3476(86)81034-4. View

Kim H, Arrobio J, BRANDT C, Jeffries B, Pyles G, Reid J . Epidemiology of respiratory syncytial virus infection in Washington, D.C. I. Importance of the virus in different respiratory tract disease syndromes and temporal distribution of infection. Am J Epidemiol. 1973; 98(3):216-25. DOI: 10.1093/oxfordjournals.aje.a121550. View

BRANDT C, Kim H, Arrobio J, Jeffries B, Wood S, Chanock R . Epidemiology of respiratory syncytial virus infection in Washington, D.C. 3. Composite analysis of eleven consecutive yearly epidemics. Am J Epidemiol. 1973; 98(5):355-64. DOI: 10.1093/oxfordjournals.aje.a121565. View

Monto A, Ullman B . Acute respiratory illness in an American community. The Tecumseh study. JAMA. 1974; 227(2):164-9. View

Minnich L, RAY C . Early testing of cell cultures for detection of hemadsorbing viruses. J Clin Microbiol. 1987; 25(2):421-2. PMC: 265912. DOI: 10.1128/jcm.25.2.421-422.1987. View

Swenson P, Kaplan M . Rapid detection of influenza virus in cell culture by indirect immunoperoxidase staining with type-specific monoclonal antibodies. Diagn Microbiol Infect Dis. 1987; 7(4):265-8. DOI: 10.1016/0732-8893(87)90142-8. View

August M, Warford A . Evaluation of a commercial monoclonal antibody for detection of adenovirus antigen. J Clin Microbiol. 1987; 25(11):2233-5. PMC: 269453. DOI: 10.1128/jcm.25.11.2233-2235.1987. View

STOUT C, Murphy M, Lawrence S, Julian S . Evaluation of a monoclonal antibody pool for rapid diagnosis of respiratory viral infections. J Clin Microbiol. 1989; 27(3):448-52. PMC: 267338. DOI: 10.1128/jcm.27.3.448-452.1989. View

10.

Anderson L, Parker R, Strikas R . Association between respiratory syncytial virus outbreaks and lower respiratory tract deaths of infants and young children. J Infect Dis. 1990; 161(4):640-6. DOI: 10.1093/infdis/161.4.640. View

11.

Matthey S, Nicholson D, Ruhs S, Alden B, Knock M, Schultz K . Rapid detection of respiratory viruses by shell vial culture and direct staining by using pooled and individual monoclonal antibodies. J Clin Microbiol. 1992; 30(3):540-4. PMC: 265105. DOI: 10.1128/jcm.30.3.540-544.1992. View

12.

Hemming V . Viral respiratory diseases in children: classification, etiology, epidemiology, and risk factors. J Pediatr. 1994; 124(5 Pt 2):S13-6. PMC: 7172644. DOI: 10.1016/s0022-3476(94)70185-7. View

13.

Fulton R, Middleton P . Comparison of immunofluorescence and isolation techniques in the diagnosis of respiratory viral infections of children. Infect Immun. 1974; 10(1):92-101. PMC: 414962. DOI: 10.1128/iai.10.1.92-101.1974. View

14.

Hall C, DOUGLAS Jr R . Clinically useful method for the isolation of respiratory syncytial virus. J Infect Dis. 1975; 131(1):1-5. DOI: 10.1093/infdis/131.1.1. View

15.

Tyeryar F, Richardson L, Belshe R . From the National Institutes of Health: report of a workshop on respiratory syncytial virus and parainfluenza viruses. J Infect Dis. 1978; 137(6):835-46. DOI: 10.1093/infdis/137.6.835. View

16.

Kim H, BRANDT C, Arrobio J, Murphy B, Chanock R, PARROTT R . Influenza A and B virus infection in infants and young children during the years 1957-1976. Am J Epidemiol. 1979; 109(4):464-79. DOI: 10.1093/oxfordjournals.aje.a112704. View

17.

Wong D, Welliver R, Riddlesberger K, Sun M, Ogra P . Rapid diagnosis of parainfluenza virus infection in children. J Clin Microbiol. 1982; 16(1):164-7. PMC: 272313. DOI: 10.1128/jcm.16.1.164-167.1982. View

18.

Glezen P, Denny F . Epidemiology of acute lower respiratory disease in children. N Engl J Med. 1973; 288(10):498-505. DOI: 10.1056/NEJM197303082881005. View