Relation of the Escherichia Coli DnaX Gene to Its Two Products--the Tau and Gamma Subunits of DNA Polymerase III Holoenzyme

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1987 Oct 12

PMID 3313272

Citations 19

Authors

S H Lee

P Kanda

R C Kennedy

J R Walker

Affiliations

Soon will be listed here.

Abstract

The Escherichia coli DNA polymerase III holoenzyme 71.1 kDa tau subunit is a 643 amino acid protein encoded by the dnaX gene. This gene also encodes the holoenzyme 56.5 kDa gamma subunit. The tau factor (as a tau'-LacZ' fusion protein) has been isolated and shown to be cleaved in vitro to form gamma and a 135 kda C-terminal cleavage product. The tau'-LacZ' fusion protein, gamma, and the C-terminal cleavage product have been isolated. N-terminal sequencing has demonstrated that tau and gamma share the same N-terminal sequences and that tau is proteolytically cleaved in vitro between residues 498 and 499 to form gamma. In addition, residues 420-440 were shown to be present in both tau and gamma by use of antibody specific for a synthetic peptide corresponding to that sequence. Some mechanism functions in vivo to ensure that tau and gamma are synthesized in a ratio of about one-to-one, as shown by radioimmune precipitation of tau and gamma from cellular extracts.

Citing Articles

Solution study of the Escherichia coli DNA polymerase III clamp loader reveals the location of the dynamic ψχ heterodimer.

Tondnevis F, Gillilan R, Bloom L, McKenna R Struct Dyn. 2016; 2(5):054701.

PMID: 26798827 PMC: 4711647. DOI: 10.1063/1.4927407.

Potential role of proteolysis in the control of UvrABC incision.

Caron P, Grossman L Nucleic Acids Res. 1988; 16(20):9641-50.

PMID: 16617483 PMC: 338769. DOI: 10.1093/nar/16.20.9641.

Linkage map of Escherichia coli K-12, edition 10: the traditional map.

Berlyn M Microbiol Mol Biol Rev. 1998; 62(3):814-984.

PMID: 9729611 PMC: 98936. DOI: 10.1128/MMBR.62.3.814-984.1998.

Conservation of the Escherichia coli dnaX programmed ribosomal frameshift signal in Salmonella typhimurium.

Blinkova A, Burkart M, Owens T, Walker J J Bacteriol. 1997; 179(13):4438-42.

PMID: 9209069 PMC: 179275. DOI: 10.1128/jb.179.13.4438-4442.1997.

Programmed translational frameshifting.

Farabaugh P Microbiol Rev. 1996; 60(1):103-34.

PMID: 8852897 PMC: 239420. DOI: 10.1128/mr.60.1.103-134.1996.

References

Kodaira M, Biswas S, Kornberg A . The dnaX gene encodes the DNA polymerase III holoenzyme tau subunit, precursor of the gamma subunit, the dnaZ gene product. Mol Gen Genet. 1983; 192(1-2):80-6. DOI: 10.1007/BF00327650. View

Mullin D, Woldringh C, Henson J, Walker J . Cloning of the Escherichia coli dnaZX region and identification of its products. Mol Gen Genet. 1983; 192(1-2):73-9. DOI: 10.1007/BF00327649. View

DiFrancesco R, Bhatnagar S, Brown A, Bessman M . The interaction of DNA polymerase III and the product of the Escherichia coli mutator gene, mutD. J Biol Chem. 1984; 259(9):5567-73. View

Walker J, Saraste M, Runswick M, Gay N . Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold. EMBO J. 1982; 1(8):945-51. PMC: 553140. DOI: 10.1002/j.1460-2075.1982.tb01276.x. View

Biswas S, Kornberg A . Nucleoside triphosphate binding to DNA polymerase III holoenzyme of Escherichia coli. A direct photoaffinity labeling study. J Biol Chem. 1984; 259(12):7990-3. View

Scheuermann R, Echols H . A separate editing exonuclease for DNA replication: the epsilon subunit of Escherichia coli DNA polymerase III holoenzyme. Proc Natl Acad Sci U S A. 1984; 81(24):7747-51. PMC: 392229. DOI: 10.1073/pnas.81.24.7747. View

McHenry C . DNA polymerase III holoenzyme of Escherichia coli: components and function of a true replicative complex. Mol Cell Biochem. 1985; 66(1):71-85. DOI: 10.1007/BF00231826. View

ODonnell M, Kornberg A . Complete replication of templates by Escherichia coli DNA polymerase III holoenzyme. J Biol Chem. 1985; 260(23):12884-9. View

Maki H, Kornberg A . The polymerase subunit of DNA polymerase III of Escherichia coli. II. Purification of the alpha subunit, devoid of nuclease activities. J Biol Chem. 1985; 260(24):12987-92. View

10.

Fry D, KUBY S, Mildvan A . ATP-binding site of adenylate kinase: mechanistic implications of its homology with ras-encoded p21, F1-ATPase, and other nucleotide-binding proteins. Proc Natl Acad Sci U S A. 1986; 83(4):907-11. PMC: 322979. DOI: 10.1073/pnas.83.4.907. View

11.

Kennedy R, Henkel R, Pauletti D, Allan J, Lee T, Essex M . Antiserum to a synthetic peptide recognizes the HTLV-III envelope glycoprotein. Science. 1986; 231(4745):1556-9. DOI: 10.1126/science.3006246. View

12.

Pauletti D, Simmonds R, Dreesman G, Kennedy R . Application of a modified computer algorithm in determining potential antigenic determinants associated with the AIDS virus glycoprotein. Anal Biochem. 1985; 151(2):540-6. DOI: 10.1016/0003-2697(85)90217-9. View

13.

Lanford R, Kanda P, Kennedy R . Induction of nuclear transport with a synthetic peptide homologous to the SV40 T antigen transport signal. Cell. 1986; 46(4):575-82. DOI: 10.1016/0092-8674(86)90883-4. View

14.

Yin K, Blinkowa A, Walker J . Nucleotide sequence of the Escherichia coli replication gene dnaZX. Nucleic Acids Res. 1986; 14(16):6541-9. PMC: 311662. DOI: 10.1093/nar/14.16.6541. View

15.

Higgins C, Hiles I, Salmond G, Gill D, Downie J, Evans I . A family of related ATP-binding subunits coupled to many distinct biological processes in bacteria. Nature. 1986; 323(6087):448-50. DOI: 10.1038/323448a0. View

16.

Flower A, McHenry C . The adjacent dnaZ and dnaX genes of Escherichia coli are contained within one continuous open reading frame. Nucleic Acids Res. 1986; 14(20):8091-101. PMC: 311836. DOI: 10.1093/nar/14.20.8091. View

17.

Chanh T, Dreesman G, Kanda P, Linette G, Sparrow J, Ho D . Induction of anti-HIV neutralizing antibodies by synthetic peptides. EMBO J. 1986; 5(11):3065-71. PMC: 1167262. DOI: 10.1002/j.1460-2075.1986.tb04607.x. View

18.

Lee S, Walker J . Escherichia coli DnaX product, the tau subunit of DNA polymerase III, is a multifunctional protein with single-stranded DNA-dependent ATPase activity. Proc Natl Acad Sci U S A. 1987; 84(9):2713-7. PMC: 304728. DOI: 10.1073/pnas.84.9.2713. View

19.

HOWARD-FLANDERS P, Simson E, THERIOT L . A LOCUS THAT CONTROLS FILAMENT FORMATION AND SENSITIVITY TO RADIATION IN ESCHERICHIA COLI K-12. Genetics. 1964; 49:237-46. PMC: 1210565. DOI: 10.1093/genetics/49.2.237. View

20.

Wickner W, Schekman R, Geider K, Kornberg A . A new form of DNA polymerase 3 and a copolymerase replicate a long, single-stranded primer-template. Proc Natl Acad Sci U S A. 1973; 70(6):1764-7. PMC: 433591. DOI: 10.1073/pnas.70.6.1764. View