Identification, Isolation, and Overexpression of the Gene Encoding the Psi Subunit of DNA Polymerase III Holoenzyme

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1993 Sep 1

PMID 8366044

Citations 6

Authors

J R Carter

M A Franden

R Aebersold

C S McHenry

Affiliations

Soon will be listed here.

Abstract

The gene encoding the psi subunit of DNA polymerase III holoenzyme, holD, was identified and isolated by an approach in which peptide sequence data were used to obtain a DNA hybridization probe. The gene, which maps to 99.3 centisomes, was sequenced and found to be identical to a previously uncharacterized open reading frame that overlaps the 5' end of rimI by 29 bases, contains 411 bp, and is predicted to encode a protein of 15,174 Da. When expressed in a plasmid that also expressed holC, holD directed expression of the psi subunit to about 3% of total soluble protein.

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