Dynamic Networks Observed in the Nucleosome Core Particles Couple the Histone Globular Domains with DNA

Overview

Journal Commun Biol

Specialty Biology

Date 2020 Oct 31

PMID 33128005

Citations 20

Authors

Xiangyan Shi

Chinmayi Prasanna

Aghil Soman

Konstantin Pervushin

Lars Nordenskiold

Affiliations

Soon will be listed here.

Abstract

The dynamics of eukaryotic nucleosomes are essential in gene activity and well regulated by various factors. Here, we elucidated the internal dynamics at multiple timescales for the human histones hH3 and hH4 in the Widom 601 nucleosome core particles (NCP), suggesting that four dynamic networks are formed by the residues exhibiting larger-scale μs-ms motions that extend from the NCP core to the histone tails and DNA. Furthermore, despite possessing highly conserved structural features, histones in the telomeric NCP exhibit enhanced μs-ms dynamics in the globular sites residing at the identified dynamic networks and in a neighboring region. In addition, higher mobility was observed for the N-terminal tails of hH3 and hH4 in the telomeric NCP. The results demonstrate the existence of dynamic networks in nucleosomes, through which the center of the core regions could interactively communicate with histone tails and DNA to potentially propagate epigenetic changes.

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