A Restriction Endonuclease Cleavage Map of Mouse Mitochondrial DNA

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1977 Jan 1

PMID 331253

Citations 11

Authors

K H Moore

P H Johnson

S E Chandler

L I Grossman

Affiliations

Soon will be listed here.

Abstract

A restriction endonuclease cleavage map is presented for mouse mitochondrial DNA. This map was constructed by electron microscopic measurements on partial digests containing fixed D-loops, and by electrophoretic analysis of partial and complete single enzyme digests, and of double digests. No map differences were detected between mitochondrial DNA from cultured LA9 cells and an inbred mouse line for the six endonucleases used. Three cleavage sites recognized by HpaI, five sites recognized by HincII, two sites recognized PstI and four sites recognized by BamI were located with respect to the origin of replication and the EcoRI and HinIII sites previously determined by others. No cleavages were produced by KpnI or SalI. The migration of linear DNA with a molecular weight greater than 1 X 10(6) was not a linear function of log molecular weight in 1% agarose gels run at 6.6 volts/cm.

Citing Articles

Organization of Achlya mtDNA: a population with two orientations and a large inverted repeat containing the rRNA genes.

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Detection and mapping of homologous, repeated and amplified DNA sequences by DNA renaturation in agarose gels.

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Heterology of mitochondrial DNA from mammals detected by electron microscopic heteroduplex analyses.

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Evolutionary relationships among five subspecies of Mus musculus based on restriction enzyme cleavage patterns of mitochondrial DNA.

Yonekawa H, Moriwaki K, Gotoh O, Hayashi J, Watanabe J, Miyashita N Genetics. 1981; 98(4):801-16.

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Achlya mitochondrial DNA: gene localization and analysis of inverted repeats.

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