Screening for Anti-Influenza Actives of Prefractionated Traditional Chinese Medicines

Overview

Journal Evid Based Complement Alternat Med

Specialty Rehabilitation Medicine

Date 2020 Oct 30

PMID 33123207

Citations 3

Authors

Qianli Kang

Yanyan Wang

Qinghua Cui

Lili Gong

Yong Yang

Haiqiang Jiang

Lijun Rong

Rong Rong

Ruikun Du

Affiliations

Soon will be listed here.

Abstract

Traditional Chinese medicines (TCMs) have proven to possess advantages in counteracting virus infections according to clinical practices. It's therefore of great value to discover novel antivirals from TCMs. In this paper, One hundred medicinal plants which have been included in TCM prescriptions for antiviral treatment were selected and prefractionated into 5 fractions each by sequentially using cyclohexane, dichloromethane, ethyl acetate, -butanol, and water. 500 TCM-simplified extracts were then subjected to a phenotypic screening using a recombinant IAV expressing Gaussia luciferase. Ten TCM fractions were identified to possess antiviral activities against influenza virus. The IC50's of the hit fractions range from 1.08 to 6.45 g/mL, while the SIs, from 7.52 to 98.40. Furthermore, all the ten hit fractions inhibited the propagation of progeny influenza virus significantly at 20 g/mL. The hit TCM fractions deserve further isolation for responsible constituents leading towards anti-influenza drugs. Moreover, a library consisting of 500 simplified TCM extracts was established, facilitating antiviral screening in quick response to emerging and re-emerging viruses such as Ebola virus and current SARS-CoV-2 pandemic.

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