Screening Antitumor Bioactive Fraction from Sauromatum Giganteum (Engl.) Cusimano & Hett and Sensitive Cell Lines with the Serum Pharmacology Method and Identification by UPLC-TOF-MS

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2015 Mar 11

PMID 25756649

Citations 4

Authors

Shi-Yong Gao

Yun-Fei Gong

Qiu-Jia Sun

Jing Bai

Long Wang

Zi-Quan Fan

Yu Sun

Yi-Jun Su

Jian Gang

Yu-Bin Ji

Affiliations

Soon will be listed here.

Abstract

Sauromatum giganteum (Engl.) Cusimano & Hett Tuber are used in Chinese folklore medicine for treatment of neoplasms. However, the claim has not been scientifically validated. The aim of the study is to screen the antitumor bioactive fraction of Sauromatum giganteum (Engl.) Cusimano & Hett Tuber and sensitive tumor cell lines using a cytotoxicity assay in vitro and tumor transplantation method in vivo, to support its use in folk medicine. The petroleum ether fraction, chloroform fraction, ethyl acetate fraction, n-butanol fraction and water fraction were successively extracted by turn by the maceration under reflux assay. Screening of antitumor bioactive fraction and sensitive cell lines were measured by MTT assay and the serum pharmacology method, and in vivo the antitumor activities of the active fraction was evaluated by using S180 or H22 tumor-bearing mice model and Kunming mice. The active constituents of ethyl acetate fraction of Sauromatum giganteum (Engl.) Cusimano & Hett were characterized by UPLC-TOF-MS. Compared with control groups, mice serum containing ethyl acetate fraction had a inhibition effect on SMMC-7721 cell, SGC-7901 cell, MCF-7 cell, HeLa cell, A549 cell, HT-29, and MDA-MB-231, respectively, but mice serum containing other four fractions had no different with that of control group. The inhibition capabilities of mice serum containing ethyl acetate fraction on the seven cell lines in descending order is SGC-7901 > SMMC-7721 > MCF-7 > HT-29 > A549 > HeLa > MDA-MB-231. In vivo the inhibition rate of 106, 318, 954 mg/kg·d ethyl acetate fraction dry extract to sarcoma S180 is 15.22%, 26.15% and 40.24%, respectively, and life prolonging rate to hepatoma H22 is 33.61%, 40.16% and 55.74%. A total of 14 compounds were identified in the ethyl acetate fraction of Sauromatum giganteum (Engl.) Cusimano & Hett. The results of the experimental studies proved the antitumor activity of Sauromatum giganteum (Engl.) Cusimano & Hett and supported the traditional use of this plant. These data indicate the potential for the use of ethyl acetate fraction of Sauromatum giganteum (Engl.) Cusimano & Hett Tuber in tumor therapy, anti-tumor activity on cancer cell line in descending order is SGC-7901 > SMMC-7721 > MCF-7 > HT-29 > A549 > HeLa > MDA-MB-231.

Citing Articles

Chemical characterization of the crude extract of Sauromatum venosum (voodoo lily) and docking study with 12-O-acetylingol 8-tiglate for cytotoxicity testing in SaOS (osteoblastic osteosarcoma cells).

Kahar N, Mishra P, Bhatt R, Seth R Anal Sci. 2023; 40(1):151-162.

PMID: 37872463 DOI: 10.1007/s44211-023-00441-2.

Screening for Anti-Influenza Actives of Prefractionated Traditional Chinese Medicines.

Kang Q, Wang Y, Cui Q, Gong L, Yang Y, Jiang H Evid Based Complement Alternat Med. 2020; 2020:4979850.

PMID: 33123207 PMC: 7584957. DOI: 10.1155/2020/4979850.

Correlation of the cytotoxic effects of cationic lipids with their headgroups.

Cui S, Wang Y, Gong Y, Lin X, Zhao Y, Zhi D Toxicol Res (Camb). 2018; 7(3):473-479.

PMID: 30090597 PMC: 6062336. DOI: 10.1039/c8tx00005k.

R-Phycoerythrin Induces SGC-7901 Apoptosis by Arresting Cell Cycle at S Phase.

Tan H, Gao S, Zhuang Y, Dong Y, Guan W, Zhang K Mar Drugs. 2016; 14(9).

PMID: 27626431 PMC: 5039537. DOI: 10.3390/md14090166.

References

Chen X, Chen D, Si J, Tu G . Chemical constituents of Typhonium giganteum Engl. J Asian Nat Prod Res. 2002; 3(4):277-83. DOI: 10.1080/10286020108040367. View

Shan B, Zhang J, Li Q . [Human T cell and monocyte modulating activity of Rhizoma typhonii in vitro]. Zhongguo Zhong Xi Yi Jie He Za Zhi. 2003; 21(10):768-72. View

Liu H, Pan C, Yang W, Liu X . Antitumor and immunomodulatory activity of resveratrol on experimentally implanted tumor of H22 in Balb/c mice. World J Gastroenterol. 2003; 9(7):1474-6. PMC: 4615485. DOI: 10.3748/wjg.v9.i7.1474. View

Tan R, Chen J . The cerebrosides. Nat Prod Rep. 2003; 20(5):509-34. DOI: 10.1039/b307243f. View

Wang S, Ni H, Cheng H, Wang G, Wang T, Chen L . [Detection of differentially expressed genes in hepatocellularcarcinoma cells SMMC-7721 treated with Typhonium giganteum extract by mRNA differential display]. Zhongguo Zhong Yao Za Zhi. 2005; 29(10):974-7. View

Bochu W, Liancai Z, Qi C . Primary study on the application of Serum Pharmacology in Chinese traditional medicine. Colloids Surf B Biointerfaces. 2005; 43(3-4):194-7. DOI: 10.1016/j.colsurfb.2005.04.013. View

Lindequist U, Niedermeyer T, Julich W . The pharmacological potential of mushrooms. Evid Based Complement Alternat Med. 2005; 2(3):285-99. PMC: 1193547. DOI: 10.1093/ecam/neh107. View

Gao S, Wang C, Chen X, Yu W, Mo B, Li C . Cerebrosides of baifuzi, a novel potential blocker of calcium-activated chloride channels in rat pulmonary artery smooth muscle cells. Cell Biol Int. 2007; 31(9):908-15. DOI: 10.1016/j.cellbi.2007.02.005. View

Corson T, Crews C . Molecular understanding and modern application of traditional medicines: triumphs and trials. Cell. 2007; 130(5):769-74. PMC: 2507744. DOI: 10.1016/j.cell.2007.08.021. View

10.

Cao Y, Xia Q, Meng H, Zhong A . Pharmacological effects of serum containing chinese medicine Bushen Huayu Jiedu Compound Recipe in lung cancer drug-resistance cells. Chin J Integr Med. 2008; 14(1):46-50. DOI: 10.1007/s11655-007-9004-4. View

11.

Montenegro R, Farias R, Pereira M, Nunes Alves A, Bezerra F, Andrade-Neto M . Antitumor activity of pisosterol in mice bearing with S180 tumor. Biol Pharm Bull. 2008; 31(3):454-7. DOI: 10.1248/bpb.31.454. View

12.

Zhang H, Xing W, Li Y, Zhu Z, Wu J, Zhang Q . Effects of a traditional Chinese herbal preparation on osteoblasts and osteoclasts. Maturitas. 2008; 61(4):334-9. DOI: 10.1016/j.maturitas.2008.09.023. View

13.

Tang D, Zhang Z, Gao Y, Wei Y, Han L . Protective effects of serum containing Ginkgo biloba extract on glomerulosclerosis in rat mesangial cells. J Ethnopharmacol. 2009; 124(1):26-33. DOI: 10.1016/j.jep.2009.04.017. View

14.

Zhang Y, Liu J, Wen B, Liu N . Mechanisms of inhibiting proliferation of vascular smooth muscle cells by serum of rats treated with Dahuang Zhechong pill. J Ethnopharmacol. 2009; 124(1):125-9. DOI: 10.1016/j.jep.2009.04.012. View

15.

Maioli E, Torricelli C, Fortino V, Carlucci F, Tommassini V, Pacini A . Critical appraisal of the MTT assay in the presence of rottlerin and uncouplers. Biol Proced Online. 2009; 11:227-40. PMC: 3056051. DOI: 10.1007/s12575-009-9020-1. View

16.

Xu H, Qi J, Wang G, Deng H, Qi Z . The effect of single cerebroside compounds on activation of BKCa channels. Mol Membr Biol. 2010; 28(2):145-54. DOI: 10.3109/09687688.2010.538731. View

17.

Chi S, Cai W, Liu P, Zhang Z, Chen X, Gao L . Baifuzi reduces transient ischemic brain damage through an interaction with the STREX domain of BKCa channels. Cell Death Dis. 2011; 1:e13. PMC: 3039290. DOI: 10.1038/cddis.2009.10. View

18.

Li X, Wu M, Ye H, Chen W, Lin J, Zheng L . Experimental study on the suppression of sodium nitroprussiate-induced chondrocyte apoptosis by Tougu Xiaotong Capsule (透骨消痛胶囊)-containing serum. Chin J Integr Med. 2011; 17(6):436-43. DOI: 10.1007/s11655-011-0751-x. View

19.

Xue R, Cao Y, Han N, Lin X, Liu Z, Yin J . Activity of DBXX granules on anti-gastric ulcer and decreasing the side effect of chemotherapy in S180 tumor-bearing mice. J Ethnopharmacol. 2011; 137(3):1156-60. DOI: 10.1016/j.jep.2011.07.042. View

20.

Li Q, Jiang C, Zu Y, Song Z, Zhang B, Meng X . SFE-CO2 extract from Typhonium giganteum Engl. tubers, induces apoptosis in human hepatoma SMMC-7721 cells involvement of a ROS-mediated mitochondrial pathway. Molecules. 2011; 16(10):8228-42. PMC: 6264752. DOI: 10.3390/molecules16108228. View