Extraction, Characterization and Incorporation of Extract in Ad Hoc Formulated Phospholipid Vesicles Designed for the Treatment of Skin Diseases Connected with Oxidative Stress

Overview

Journal Pharmaceutics

Publisher MDPI

Date 2020 Oct 29

PMID 33113923

Citations 3

Authors

Mohamad Allaw

Maria Manconi

Matteo Aroffu

Francesca Marongiu

Marco Porceddu

Gianluigi Bacchetta

Iris Usach

Rita Abi Rached

Hiba N Rajha

Richard G Maroun

Jose Luis Pedraz

Tania B Lopez-Mendez

Anna Maria Fadda

Maria Letizia Manca

Affiliations

Soon will be listed here.

Abstract

An extract of an endangered endemic plant of Sardinia (Italy), was prepared and characterized. It was loaded in special phospholipid vesicles, glycerosomes, which were modified by adding maltodextrin (glucidex) and a polymer (gelatin or hyaluronan). The corresponding liposomes were also prepared and used as reference. The vesicles disclosed suitable physicochemical features for skin delivery. Indeed, their mean diameter ranged from 120 to 160 nm, they were homogeneously dispersed (polydispersity index ≤ 0.30), and their zeta potential was highly negative (~-45 mV). The vesicle dispersions maintained unchanged characteristics during 60 days of storage, were highly biocompatible, and were able to protect keratinocytes against damages due to oxidative stress induced by treating them with hydrogen peroxide. Vesicles were also capable of promoting cell proliferation and migration in vitro by means of a scratch wound assay. The results confirmed the fruitful delivery of the extract of in glycerosomes modified with glucidex and gelatin and their promising ability for skin protection and treatment.

Citing Articles

Extraction, Physicochemical Properties, Anti-Aging, and Antioxidant Activities of Polysaccharides from Industrial Hemp Residues.

Chang T, Li H, Lv H, Tan M, Hou S, Liu X Molecules. 2022; 27(18).

PMID: 36144481 PMC: 9503781. DOI: 10.3390/molecules27185746.

Vesicular Nanocarriers for Phytocompounds in Wound Care: Preparation and Characterization.

Safta D, Bogdan C, Moldovan M Pharmaceutics. 2022; 14(5).

PMID: 35631577 PMC: 9147886. DOI: 10.3390/pharmaceutics14050991.

Jabuticaba () Peel as a Sustainable Source of Anthocyanins and Ellagitannins Delivered by Phospholipid Vesicles for Alleviating Oxidative Stress in Human Keratinocytes.

Castangia I, Manca M, Allaw M, Hellstrom J, Granato D, Manconi M Molecules. 2021; 26(21).

PMID: 34771107 PMC: 8587422. DOI: 10.3390/molecules26216697.

References

Birch-Machin M, Swalwell H . How mitochondria record the effects of UV exposure and oxidative stress using human skin as a model tissue. Mutagenesis. 2009; 25(2):101-7. DOI: 10.1093/mutage/gep061. View

Cristiano M, Froiio F, Spaccapelo R, Mancuso A, Nistico S, Udongo B . Sulforaphane-Loaded Ultradeformable Vesicles as A Potential Natural Nanomedicine for the Treatment of Skin Cancer Diseases. Pharmaceutics. 2019; 12(1). PMC: 7023209. DOI: 10.3390/pharmaceutics12010006. View

Derycke A, de Witte P . Transferrin-mediated targeting of hypericin embedded in sterically stabilized PEG-liposomes. Int J Oncol. 2001; 20(1):181-7. View

Castangia I, Manca M, Matricardi P, Sinico C, Lampis S, Fernandez-Busquets X . Effect of diclofenac and glycol intercalation on structural assembly of phospholipid lamellar vesicles. Int J Pharm. 2013; 456(1):1-9. DOI: 10.1016/j.ijpharm.2013.08.034. View

Genc Y, Guragac Dereli F, Saracoglu I, Akkol E . The inhibitory effects of isolated constituents from subsp. L. on collagenase, elastase and hyaluronidase enzymes: Potential wound healer. Saudi Pharm J. 2020; 28(1):101-106. PMC: 6950955. DOI: 10.1016/j.jsps.2019.11.011. View

Sanna C, Scognamiglio M, Fiorentino A, Corona A, Graziani V, Caredda A . Prenylated phloroglucinols from Hypericum scruglii, an endemic species of Sardinia (Italy), as new dual HIV-1 inhibitors effective on HIV-1 replication. PLoS One. 2018; 13(3):e0195168. PMC: 5877874. DOI: 10.1371/journal.pone.0195168. View

Zhang X, Ye Y, Xia F, Yang X, Xu G . Diverse Polyphenols from Hypericum faberi. Nat Prod Bioprospect. 2019; 9(3):215-221. PMC: 6538727. DOI: 10.1007/s13659-019-0206-1. View

Castangia I, Caddeo C, Manca M, Casu L, Latorre A, Diez-Sales O . Delivery of liquorice extract by liposomes and hyalurosomes to protect the skin against oxidative stress injuries. Carbohydr Polym. 2015; 134:657-63. DOI: 10.1016/j.carbpol.2015.08.037. View

Manconi M, Mura S, Manca M, Fadda A, Dolz M, Hernandez M . Chitosomes as drug delivery systems for C-phycocyanin: preparation and characterization. Int J Pharm. 2010; 392(1-2):92-100. DOI: 10.1016/j.ijpharm.2010.03.038. View

10.

Mir-Palomo S, Nacher A, Diez-Sales O, Ofelia Vila Buso M, Caddeo C, Manca M . Inhibition of skin inflammation by baicalin ultradeformable vesicles. Int J Pharm. 2016; 511(1):23-29. DOI: 10.1016/j.ijpharm.2016.06.136. View

11.

Mandrone M, Scognamiglio M, Fiorentino A, Sanna C, Cornioli L, Antognoni F . Phytochemical profile and α-glucosidase inhibitory activity of Sardinian Hypericum scruglii and Hypericum hircinum. Fitoterapia. 2017; 120:184-193. DOI: 10.1016/j.fitote.2017.06.020. View

12.

Suntar I, Akkol E, Yilmazer D, Baykal T, Kirmizibekmez H, Alper M . Investigations on the in vivo wound healing potential of Hypericum perforatum L. J Ethnopharmacol. 2009; 127(2):468-77. DOI: 10.1016/j.jep.2009.10.011. View

13.

Orcic D, Mimica-Dukic N, Franciskovic M, Petrovic S, Jovin E . Antioxidant activity relationship of phenolic compounds in Hypericum perforatum L. Chem Cent J. 2011; 5:34. PMC: 3132159. DOI: 10.1186/1752-153X-5-34. View

14.

Chua L, Lee S, Abdullah N, Sarmidi M . Review on Labisia pumila (Kacip Fatimah): bioactive phytochemicals and skin collagen synthesis promoting herb. Fitoterapia. 2012; 83(8):1322-35. DOI: 10.1016/j.fitote.2012.04.002. View

15.

Hu F, Yan Y, Wang C, Liu Y, Wang J, Zhou F . Article Effect and Mechanism of Ganoderma lucidum Polysaccharides on Human Fibroblasts and Skin Wound Healing in Mice. Chin J Integr Med. 2018; 25(3):203-209. DOI: 10.1007/s11655-018-3060-9. View

16.

Church D, Elsayed S, Reid O, Winston B, Lindsay R . Burn wound infections. Clin Microbiol Rev. 2006; 19(2):403-34. PMC: 1471990. DOI: 10.1128/CMR.19.2.403-434.2006. View

17.

Yue P, Leung E, Mak N, Wong R . A simplified method for quantifying cell migration/wound healing in 96-well plates. J Biomol Screen. 2010; 15(4):427-33. DOI: 10.1177/1087057110361772. View

18.

Scalia S, Franceschinis E, Bertelli D, Iannuccelli V . Comparative evaluation of the effect of permeation enhancers, lipid nanoparticles and colloidal silica on in vivo human skin penetration of quercetin. Skin Pharmacol Physiol. 2012; 26(2):57-67. DOI: 10.1159/000345210. View

19.

Moolakkadath T, Aqil M, Ahad A, Imam S, Praveen A, Sultana Y . Preparation and optimization of fisetin loaded glycerol based soft nanovesicles by Box-Behnken design. Int J Pharm. 2020; 578:119125. DOI: 10.1016/j.ijpharm.2020.119125. View

20.

Plenagl N, Seitz B, Duse L, Pinnapireddy S, Jedelska J, Brussler J . Hypericin inclusion complexes encapsulated in liposomes for antimicrobial photodynamic therapy. Int J Pharm. 2019; 570:118666. DOI: 10.1016/j.ijpharm.2019.118666. View