Circular RNA Hsa_circ_0001785 Inhibits the Proliferation, Migration and Invasion of Breast Cancer Cells in Vitro and in Vivo by Sponging MiR-942 to Upregulate SOCS3

Overview

Journal Cell Cycle

Specialty Cell Biology

Date 2020 Oct 15

PMID 33054543

Citations 17

Authors

Zhiyang Li

Jiehua Zheng

Weixun Lin

Jiaquan Weng

Weida Hong

Juan Zou

Taofeng Zhang

Changsheng Ye

Yexi Chen

Affiliations

Soon will be listed here.

Abstract

Circular RNAs (circRNAs) are a class of widely expressed noncoding RNA with significant regulatory potential discovered in recent years. The purpose of this study was to investigate the effects of hsa_circ_0001785 on the proliferation, migration and invasion of breast cancer (BC) cells and and the potential underlying molecular mechanism. In the present study, the expressions of hsa_circ_0001785 in five BC cells (T47D, MCF-7, MDA-MB-453, MDA-MB-231 and BT-549) and one normal breast cell (MCF-10A) were the first to examined by qRT-PCR. Then, we studied the biological function of hsa_circ_0001785 in BC by and experiments. CCK-8, clone formation, wound-healing and Transwell assays were performed to analyze the cellular proliferation, migration and invasion . The subcutaneous tumor model of nude mice was used for experiment. In addition, we determined that hsa_circ_0001785 acted as competing endogenous RNAs (ceRNAs) in BC by RNA immunoprecipitation (RIP) and dual-luciferase reporter assays. Results showed that the expressions of hsa_circ_0001785 were decreased in BC cells. Hsa_circ_0001785 overexpression inhibited the proliferation, migration, invasion of BC cells and tumor growth in nude mice. RIP and dual-luciferase reporter assay demonstrated that hsa_circ_0001785 could regulate the SOCS3 by sponging miR-942. In general, circular RNA hsa_circ_0001785 inhibits the proliferation, migration and invasion of BC cells by modulating the miR-942/SOCS3 signaling axis.

Citing Articles

Development of a prognostic model based on the ceRNA network in Triple-Negative Breast cancer.

Zhu Y, Wang J, Xu B PeerJ. 2025; 13:e19063.

PMID: 40034665 PMC: 11874946. DOI: 10.7717/peerj.19063.

Circular RNAs in Cell Cycle Regulation of Cancers.

Thamjamrassri P, Ariyachet C Int J Mol Sci. 2024; 25(11).

PMID: 38892280 PMC: 11173060. DOI: 10.3390/ijms25116094.

Ensemble-based classification using microRNA expression identifies a breast cancer patient subgroup with an ultralow long-term risk of metastases.

Block I, Burton M, Sorensen K, Larsen M, Do T, Bak M Cancer Med. 2024; 13(9):e7089.

PMID: 38676390 PMC: 11053369. DOI: 10.1002/cam4.7089.

Plasma Circular RNAs as Biomarkers for Breast Cancer.

Tierno D, Grassi G, Zanconati F, Dapas B, Scaggiante B Biomedicines. 2024; 12(4).

PMID: 38672229 PMC: 11048241. DOI: 10.3390/biomedicines12040875.

Tumor Cell-Derived Exosomal miR-191-5p Activates M2-Subtype Macrophages Through SOCS3 to Facilitate Breast Cancer.

Ni J, Xi X, Xiao S, Xiao X Mol Biotechnol. 2024; 66(5):1314-1325.

PMID: 38270757 DOI: 10.1007/s12033-023-01034-0.

References

Harbeck N, Penault-Llorca F, Cortes J, Gnant M, Houssami N, Poortmans P . Breast cancer. Nat Rev Dis Primers. 2019; 5(1):66. DOI: 10.1038/s41572-019-0111-2. View

Hua Z, Ma K, Liu S, Yue Y, Cao H, Li Z . LncRNA ZEB1-AS1 facilitates ox-LDL-induced damage of HCtAEC cells and the oxidative stress and inflammatory events of THP-1 cells via miR-942/HMGB1 signaling. Life Sci. 2020; 247:117334. DOI: 10.1016/j.lfs.2020.117334. View

Xie J, Wang S, Li G, Zhao X, Jiang F, Liu J . circEPSTI1 regulates ovarian cancer progression via decoying miR-942. J Cell Mol Med. 2019; 23(5):3597-3602. PMC: 6484424. DOI: 10.1111/jcmm.14260. View

Lu L, Sun J, Shi P, Kong W, Xu K, He B . Identification of circular RNAs as a promising new class of diagnostic biomarkers for human breast cancer. Oncotarget. 2017; 8(27):44096-44107. PMC: 5546465. DOI: 10.18632/oncotarget.17307. View

Wang N, Gu Y, Li L, Wang F, Lv P, Xiong Y . Circular RNA circMYO9B facilitates breast cancer cell proliferation and invasiveness via upregulating FOXP4 expression by sponging miR-4316. Arch Biochem Biophys. 2018; 653:63-70. DOI: 10.1016/j.abb.2018.04.017. View

Yin W, Yan M, Fang X, Guo J, Xiong W, Zhang R . Circulating circular RNA hsa_circ_0001785 acts as a diagnostic biomarker for breast cancer detection. Clin Chim Acta. 2017; 487:363-368. DOI: 10.1016/j.cca.2017.10.011. View

Lai Z, Yang Y, Yan Y, Li T, Li Y, Wang Z . Analysis of co-expression networks for circular RNAs and mRNAs reveals that circular RNAs hsa_circ_0047905, hsa_circ_0138960 and has-circRNA7690-15 are candidate oncogenes in gastric cancer. Cell Cycle. 2017; 16(23):2301-2311. PMC: 5788484. DOI: 10.1080/15384101.2017.1380135. View

Bartel D . Metazoan MicroRNAs. Cell. 2018; 173(1):20-51. PMC: 6091663. DOI: 10.1016/j.cell.2018.03.006. View

Yu J, Yang M, Zhou B, Luo J, Zhang Z, Zhang W . CircRNA-104718 acts as competing endogenous RNA and promotes hepatocellular carcinoma progression through microRNA-218-5p/TXNDC5 signaling pathway. Clin Sci (Lond). 2019; 133(13):1487-1503. DOI: 10.1042/CS20190394. View

10.

Sasi W, Jiang W, Sharma A, Mokbel K . Higher expression levels of SOCS 1,3,4,7 are associated with earlier tumour stage and better clinical outcome in human breast cancer. BMC Cancer. 2010; 10:178. PMC: 2876081. DOI: 10.1186/1471-2407-10-178. View

11.

Huang X, Xie X, Wang H, Xiao X, Yang L, Tian Z . PDL1 And LDHA act as ceRNAs in triple negative breast cancer by regulating miR-34a. J Exp Clin Cancer Res. 2017; 36(1):129. PMC: 5602941. DOI: 10.1186/s13046-017-0593-2. View

12.

McAnena P, McGuire A, Ramli A, Curran C, Malone C, McLaughlin R . Breast cancer subtype discordance: impact on post-recurrence survival and potential treatment options. BMC Cancer. 2018; 18(1):203. PMC: 5819681. DOI: 10.1186/s12885-018-4101-7. View

13.

Xu J, Shao C, Wang X, Zhao X, Chen J, Ouyang Y . circTADA2As suppress breast cancer progression and metastasis via targeting miR-203a-3p/SOCS3 axis. Cell Death Dis. 2019; 10(3):175. PMC: 6382814. DOI: 10.1038/s41419-019-1382-y. View

14.

Pichler M, Calin G . MicroRNAs in cancer: from developmental genes in worms to their clinical application in patients. Br J Cancer. 2015; 113(4):569-73. PMC: 4647691. DOI: 10.1038/bjc.2015.253. View

15.

Wang H, Xiao Y, Wu L, Ma D . Comprehensive circular RNA profiling reveals the regulatory role of the circRNA-000911/miR-449a pathway in breast carcinogenesis. Int J Oncol. 2018; 52(3):743-754. PMC: 5807038. DOI: 10.3892/ijo.2018.4265. View

16.

Zhang J, Zhang Z, Sun J, Ma Q, Zhao W, Chen X . MiR-942 regulates the function of breast cancer cell by targeting FOXA2. Biosci Rep. 2019; 39(11). PMC: 6879377. DOI: 10.1042/BSR20192298. View

17.

Hu W, Tan C, He Y, Zhang G, Xu Y, Tang J . Functional miRNAs in breast cancer drug resistance. Onco Targets Ther. 2018; 11:1529-1541. PMC: 5865556. DOI: 10.2147/OTT.S152462. View

18.

Adeloye D, Sowunmi O, Jacobs W, David R, Adeosun A, Amuta A . Estimating the incidence of breast cancer in Africa: a systematic review and meta-analysis. J Glob Health. 2018; 8(1):010419. PMC: 5903682. DOI: 10.7189/jogh.08.010419. View

19.

Power E, Chin M, Haq M . Breast Cancer Incidence and Risk Reduction in the Hispanic Population. Cureus. 2018; 10(2):e2235. PMC: 5919763. DOI: 10.7759/cureus.2235. View

20.

Zhang H, Jiang L, Sun D, Hou J, Ji Z . CircRNA: a novel type of biomarker for cancer. Breast Cancer. 2017; 25(1):1-7. DOI: 10.1007/s12282-017-0793-9. View