The Mitotic Protein NuMA Plays a Spindle-independent Role in Nuclear Formation and Mechanics

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 2020 Oct 12

PMID 33044554

Citations 8

Authors

Andrea Serra-Marques

Ronja Houtekamer

Dorine Hintzen

John T Canty

Ahmet Yildiz

Sophie Dumont

Affiliations

Soon will be listed here.

Abstract

Eukaryotic cells typically form a single, round nucleus after mitosis, and failures to do so can compromise genomic integrity. How mammalian cells form such a nucleus remains incompletely understood. NuMA is a spindle protein whose disruption results in nuclear fragmentation. What role NuMA plays in nuclear integrity, and whether its perceived role stems from its spindle function, are unclear. Here, we use live imaging to demonstrate that NuMA plays a spindle-independent role in forming a single, round nucleus. NuMA keeps the decondensing chromosome mass compact at mitotic exit and promotes a mechanically robust nucleus. NuMA's C terminus binds DNA in vitro and chromosomes in interphase, while its coiled-coil acts as a central regulatory and structural element: it prevents NuMA from binding chromosomes at mitosis, regulates its nuclear mobility, and is essential for nuclear formation. Thus, NuMA plays a structural role over the cell cycle, building and maintaining the spindle and nucleus, two of the cell's largest structures.

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