Condensational Particle Growth Device for Reliable Cell Exposure at the Air-liquid Interface to Nanoparticles

Overview

Journal Aerosol Sci Technol

Date 2020 Oct 9

PMID 33033421

Citations 6

Authors

Trevor B Tilly

Ryan X Ward

Jiva K Luthra

Sarah Robinson

Arantzazu Eiguren-Fernandez

Gregory S Lewis

Richard L Salisbury

John A Lednicky

Tara L Sabo-Attwood

Saber M Hussain

Chang-Yu Wu

Affiliations

Soon will be listed here.

Abstract

A first-of-its-kind aerosol exposure device for toxicity testing, referred to as the Dosimetric Aerosol Inhalation Device (DAVID), was evaluated for its ability to deliver airborne nanoparticles to lung cells grown as air-liquid interface (ALI) cultures. For inhalation studies, ALI lung cell cultures exposed to airborne nanoparticles have more relevancy than the same cells exposed in submerged culture because ALI culture better represents the respiratory physiology and consequently more closely reflect cellular response to aerosol exposure. In DAVID, water condensation grows particles as small as 5 nm to droplets sized > 5 μm for inertial deposition at low flow rates. The application of DAVID for nanotoxicity analysis was evaluated by measuring the amount and variability in the deposition of uranine nanoparticles and then assessing the viability of ALI cell cultures exposed to clean-air under the same operational conditions. The results showed a low coefficient of variation, < 0.25, at most conditions, and low variability in deposition between the exposure wells, trials, and operational flow rates. At an operational flow rate of 4 LPM, no significant changes in cell viability were observed, and minimal effects observed at 6 LPM. The reliable and gentle deposition mechanism of DAVID makes it advantageous for nanoparticle exposure.

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