Molecular Characterization of Clinical and Environmental Vibrio Parahaemolyticus Isolates in Huzhou, China

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2020 Oct 2

PMID 33007026

Citations 5

Authors

Wei Yan

Lei Ji

Deshun Xu

Liping Chen

Xiaofang Wu

Affiliations

Soon will be listed here.

Abstract

Vibrio parahaemolyticus is responsible for seafood-borne gastroenteritis worldwide. Isolates of V. parahaemolyticus from clinical samples (n = 54) and environmental samples (n = 38) in Huzhou were analyzed by serological typing, virulence gene detection, antibiotic resistance testing, and pulsed-field gel electrophoresis (PFGE) for molecular typing. O3:K6 was the main serotype and tlh+tdh+trh- was the most frequently detected virulence genotype in clinical strains. O2:Kut was the main serotype and tlh+tdh-trh- was the most frequently detected virulence genotype in environmental strains. Antibiotic resistance testing indicated that the isolates were highly resistant to ampicillin (90.76%), followed by gentamicin and tetracycline. Following the restriction enzyme NotI digestion, the 91 strains yielded 81 PFGE patterns, and 16 clones had similarity values of > 85.00%, indicating a high level of diversity. Finally, there may be cross-contamination between freshwater and seawater products, so it is necessary to strengthen supervision of food processing.

Citing Articles

Antimicrobial resistance and genomic analysis of isolates from foodborne outbreaks, Huzhou, China, 2019-2023.

Yan W, Ji L, Dong F, Chen L, Yuan R, Zhang P Front Microbiol. 2024; 15:1439522.

PMID: 39323890 PMC: 11422088. DOI: 10.3389/fmicb.2024.1439522.

Assessment of Vibrionaceae prevalence in seafood from Qidong market and analysis of Vibrio parahaemolyticus strains.

Huang Q, Zhang Y, Zhang M, Li X, Wang Q, Ji X PLoS One. 2024; 19(8):e0309304.

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Antibiotic Resistance and Epidemiology of from Clinical Samples in Nantong, China, 2018-2021.

Huang A, Wang Y, Xu H, Jin X, Yan B, Zhang W Infect Drug Resist. 2023; 16:7413-7425.

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Pathogenic characteristics of the Vibrio parahaemolyticus which caused a gastroenteritis outbreak event in Huzhou.

Wu X, Zhu Y, Yan W, Zhang P, Chen L FEMS Microbiol Lett. 2023; 371.

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Characterization of Vibrio parahaemolyticus isolated from stool specimens of diarrhea patients in Nantong, Jiangsu, China during 2018-2020.

Sun J, Li X, Hu Z, Xue X, Zhang M, Wu Q PLoS One. 2022; 17(8):e0273700.

PMID: 36018831 PMC: 9416985. DOI: 10.1371/journal.pone.0273700.

References

Joseph S, Colwell R, Kaper J . Vibrio parahaemolyticus and related halophilic Vibrios. Crit Rev Microbiol. 1982; 10(1):77-124. DOI: 10.3109/10408418209113506. View

Bej A, Patterson D, Brasher C, Vickery M, Jones D, Kaysner C . Detection of total and hemolysin-producing Vibrio parahaemolyticus in shellfish using multiplex PCR amplification of tl, tdh and trh. J Microbiol Methods. 1999; 36(3):215-25. DOI: 10.1016/s0167-7012(99)00037-8. View

McLaughlin J, DePaola A, Bopp C, Martinek K, Napolilli N, Allison C . Outbreak of Vibrio parahaemolyticus gastroenteritis associated with Alaskan oysters. N Engl J Med. 2005; 353(14):1463-70. DOI: 10.1056/NEJMoa051594. View

Hunter S, Vauterin P, Lambert-Fair M, Van Duyne M, Kubota K, Graves L . Establishment of a universal size standard strain for use with the PulseNet standardized pulsed-field gel electrophoresis protocols: converting the national databases to the new size standard. J Clin Microbiol. 2005; 43(3):1045-50. PMC: 1081233. DOI: 10.1128/JCM.43.3.1045-1050.2005. View

Zhang H, Sun S, Shi W, Cui L, Gu Q . Serotype, virulence, and genetic traits of foodborne and clinical Vibrio parahaemolyticus isolates in Shanghai, China. Foodborne Pathog Dis. 2013; 10(9):796-804. DOI: 10.1089/fpd.2012.1378. View

Jung S . A foodborne outbreak of gastroenteritis caused by Vibrio parahaemolyticus associated with cross-contamination from squid in Korea. Epidemiol Health. 2019; 40:e2018056. PMC: 6367201. DOI: 10.4178/epih.e2018056. View

Parsons M, Cooper K, Kubota K, Puhr N, Simington S, Calimlim P . PulseNet USA standardized pulsed-field gel electrophoresis protocol for subtyping of Vibrio parahaemolyticus. Foodborne Pathog Dis. 2007; 4(3):285-92. DOI: 10.1089/fpd.2007.0089. View

Bag P, Nandi S, Bhadra R, Ramamurthy T, Bhattacharya S, Nishibuchi M . Clonal diversity among recently emerged strains of Vibrio parahaemolyticus O3:K6 associated with pandemic spread. J Clin Microbiol. 1999; 37(7):2354-7. PMC: 85163. DOI: 10.1128/JCM.37.7.2354-2357.1999. View

Tenover F, Arbeit R, Goering R, Mickelsen P, Murray B, Persing D . Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol. 1995; 33(9):2233-9. PMC: 228385. DOI: 10.1128/jcm.33.9.2233-2239.1995. View

10.

Lida T, Park K, Suthienkul O, Kozawa J, Yamaichi Y, Yamamoto K . Close proximity of the tdh, trh and ure genes on the chromosome of Vibrio parahaemolyticus. Microbiology (Reading). 1998; 144 ( Pt 9):2517-2523. DOI: 10.1099/00221287-144-9-2517. View

11.

Wang H, Tang X, Su Y, Chen J, Yan J . Characterization of clinical Vibrio parahaemolyticus strains in Zhoushan, China, from 2013 to 2014. PLoS One. 2017; 12(7):e0180335. PMC: 5498046. DOI: 10.1371/journal.pone.0180335. View

12.

Chen H, Zhou H, Zai S, Cai J, Qian F, Ma L . [The infection and molecular characteristics of Vibrio parahaemolyticus isolated from intestinal outpatient in two sentinel hospitals in Shanghai, 2010-2012]. Zhonghua Yu Fang Yi Xue Za Zhi. 2015; 49(3):233-6. View

13.

Yadav J, Das S, Dhaka P, Vijay D, Kumar M, Mukhopadhyay A . Molecular characterization and antimicrobial resistance profile of Clostridium perfringens type A isolates from humans, animals, fish and their environment. Anaerobe. 2017; 47:120-124. DOI: 10.1016/j.anaerobe.2017.05.009. View

14.

Liao Y, Li Y, Wu S, Mou J, Xu Z, Cui R . Risk Factors for Vibrio parahaemolyticus Infection in a Southern Coastal Region of China. Foodborne Pathog Dis. 2015; 12(11):881-6. PMC: 4675456. DOI: 10.1089/fpd.2015.1988. View