Recent Developments In Recombinant Proteins for Diagnosis of Human Fascioliasis

Overview

Journal Acta Parasitol

Specialty Parasitology

Date 2020 Sep 25

PMID 32974849

Citations 3

Authors

Abolfazl Mirzadeh

Farid Jafarihaghighi

Elham Kazemirad

Shokouh Shahrokhi Sabzevar

Mohammad Hossein Tanipour

Mehdi Ardjmand

Affiliations

Soon will be listed here.

Abstract

Fascioliasis is an important neglected tropical disease that causes severe injury to the bile ducts and liver. Therefore, a rapid and accurate method for detection of Fasciola hepatica infection plays a vital role in early treatment. Currently, the diagnosis of fascioliasis is mainly conducted via serological tests using the excretory/secretory (E/S) products, which might cross-react with antigens from other helminth parasitic diseases. Hence, the development of serodiagnosis test using recombinant antigens may contribute to differentiate fascioliasis from other helminth infections. In the past 20 years, many attempts have been made to exert different F. hepatica recombinant antigens to obtain a well-established standard assay with high accuracy. In this review, we address recent studies that refer to the development of serodiagnosis tests for diagnosis of human fascioliasis based on the candidate recombinant antigens produced by different approaches. Meanwhile, in the present review, some main factors have been highlighted to improve the accuracy of diagnostic tests such as the effect of refolding methods to recover antigens' tertiary structure as well as applying a mixture of recombinant antigens with the highest sensitivity and specificity to improve the accuracy of diagnostic tests.

Citing Articles

Diagnosis of Human Trematode Infections.

Esteban J, Munoz-Antoli C, Toledo R, Ash L Adv Exp Med Biol. 2024; 1454:541-582.

PMID: 39008275 DOI: 10.1007/978-3-031-60121-7_14.

Comparative evaluation of real-time PCR and ELISA for the detection of human fascioliasis.

Bakhshipour F, Zibaei M, Rokni M, Miahipour A, Firoozeh F, Beheshti M Sci Rep. 2024; 14(1):3865.

PMID: 38366006 PMC: 10873325. DOI: 10.1038/s41598-024-54602-y.

Evaluation of Semi-Nested PCR Compared with Indirect-ELISA to Diagnose Human Fasciolosis.

Alizadeh G, Aryaeipour M, Mohebali M, Mowlavi G, Raissi V, Rokni M Iran J Public Health. 2022; 51(3):686-694.

PMID: 35865068 PMC: 9276597. DOI: 10.18502/ijph.v51i3.8947.

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