Characterization and Differential Expression of a Ferritin Protein from Fasciola Hepatica

Overview

Journal Mol Biochem Parasitol

Specialties Biochemistry
Molecular Biology
Parasitology

Date 2012 Jan 14

PMID 22240114

Citations 8

Authors

Kimberly Caban-Hernandez

Jose F Gaudier

Ana M Espino

Affiliations

Soon will be listed here.

Abstract

Ferritins are proteins that play a central role in maintaining intracellular iron balance. A cDNA clone of Fasciola hepatica (687 bp long) encoding a putative 228-amino acid polypeptide (FhFtn-1) homologous with ferritins of vertebrates and invertebrates was identified. FhFtn-1 contains a conserved motif of the ferroxidase center typical of vertebrate ferritins. Phylogenetic tree analysis showed that FhFtn-1 clusters with two ferritins of Paragonimus westermani, which suggests a common ancestry for the ferritins of these two trematodes. Recombinant FhFtn-1 protein expressed and purified from an Escherichia coli system showed iron-uptake ability. Moreover, FhFtn-1 showed strong reactivity with sera from rabbits infected with F. hepatica for 2-12 weeks, which suggests that this protein could be a potential antigen for immunodiagnosis of fascioliasis. qPCR analysis demonstrated that FhFtn-1-mRNA is expressed at significantly higher levels in adults and unembryonated eggs than in juveniles or miracidia. These results represent the first characterization of a ferritin protein from the liver fluke F. hepatica.

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References

Castresana J . Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Mol Biol Evol. 2000; 17(4):540-52. DOI: 10.1093/oxfordjournals.molbev.a026334. View

Levi S, Luzzago A, Cesareni G, Cozzi A, Franceschinelli F, Albertini A . Mechanism of ferritin iron uptake: activity of the H-chain and deletion mapping of the ferro-oxidase site. A study of iron uptake and ferro-oxidase activity of human liver, recombinant H-chain ferritins, and of two H-chain deletion mutants. J Biol Chem. 1988; 263(34):18086-92. View

Sayle R . RASMOL: biomolecular graphics for all. Trends Biochem Sci. 1995; 20(9):374. DOI: 10.1016/s0968-0004(00)89080-5. View

Mohamed W, Sethi S, Darji A, Mraheil M, Hain T, Chakraborty T . Antibody targeting the ferritin-like protein controls Listeria infection. Infect Immun. 2010; 78(7):3306-14. PMC: 2897390. DOI: 10.1128/IAI.00210-10. View

Glanfield A, McManus D, Smyth D, Lovas E, Loukas A, Gobert G . A cytochrome b561 with ferric reductase activity from the parasitic blood fluke, Schistosoma japonicum. PLoS Negl Trop Dis. 2010; 4(11):e884. PMC: 2982821. DOI: 10.1371/journal.pntd.0000884. View

Yevenes A, Marquez V, Watt R . Cloning and characterization of Chlorobium tepidum ferritin. Biochimie. 2010; 93(2):352-60. DOI: 10.1016/j.biochi.2010.10.008. View

HANNA R . Fasciola hepatica: autoradiography of protein synthesis, transport, and secretion by the tegument. Exp Parasitol. 1980; 50(3):297-304. DOI: 10.1016/0014-4894(80)90033-8. View

Page R . TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci. 1996; 12(4):357-8. DOI: 10.1093/bioinformatics/12.4.357. View

Kim T, Joo I, Kang S, Cho S, Hong S . Paragonimus westermani: molecular cloning, expression, and characterization of a recombinant yolk ferritin. Exp Parasitol. 2003; 102(3-4):194-200. DOI: 10.1016/s0014-4894(03)00057-2. View

10.

von Darl M, Harrison P, Bottke W . cDNA cloning and deduced amino acid sequence of two ferritins: soma ferritin and yolk ferritin, from the snail Lymnaea stagnalis L. Eur J Biochem. 1994; 222(2):353-66. DOI: 10.1111/j.1432-1033.1994.tb18874.x. View

11.

Benitez L, HARRISON L, Parkhouse R, Garate T . Sequence and immunogenicity of Taenia saginata ferritin. Mol Biochem Parasitol. 1996; 82(1):113-6. DOI: 10.1016/0166-6851(96)02713-2. View

12.

Salimi-Bejestani M, Daniel R, Felstead S, Cripps P, Mahmoody H, Williams D . Prevalence of Fasciola hepatica in dairy herds in England and Wales measured with an ELISA applied to bulk-tank milk. Vet Rec. 2005; 156(23):729-31. DOI: 10.1136/vr.156.23.729. View

13.

Schussler P, Potters E, Winnen R, Bottke W, Kunz W . An isoform of ferritin as a component of protein yolk platelets in Schistosoma mansoni. Mol Reprod Dev. 1995; 41(3):325-30. DOI: 10.1002/mrd.1080410307. View

14.

Kelley L, Sternberg M . Protein structure prediction on the Web: a case study using the Phyre server. Nat Protoc. 2009; 4(3):363-71. DOI: 10.1038/nprot.2009.2. View

15.

Dietzel J, Hirzmann J, Preis D, Symmons P, Kunz W . Ferritins of Schistosoma mansoni: sequence comparison and expression in female and male worms. Mol Biochem Parasitol. 1992; 50(2):245-54. DOI: 10.1016/0166-6851(92)90221-5. View

16.

Felsenstein J . Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol. 1981; 17(6):368-76. DOI: 10.1007/BF01734359. View

17.

Carrano C, Bohnke R, Matzanke B . Fungal ferritins: the ferritin from mycelia of Absidia spinosa is a bacterioferritin. FEBS Lett. 1996; 390(3):261-4. DOI: 10.1016/0014-5793(96)00667-9. View

18.

Jones M, McManus D, Sivadorai P, Glanfield A, Moertel L, Belli S . Tracking the fate of iron in early development of human blood flukes. Int J Biochem Cell Biol. 2007; 39(9):1646-58. PMC: 2758302. DOI: 10.1016/j.biocel.2007.04.017. View

19.

Rodriguez-Perez J, Rodriguez-Medina J, Garcia-Blanco M, Hillyer G . Fasciola hepatica: molecular cloning, nucleotide sequence, and expression of a gene encoding a polypeptide homologous to a Schistosoma mansoni fatty acid-binding protein. Exp Parasitol. 1992; 74(4):400-7. DOI: 10.1016/0014-4894(92)90202-l. View

20.

Mas-Coma S . Epidemiology of fascioliasis in human endemic areas. J Helminthol. 2005; 79(3):207-16. DOI: 10.1079/joh2005296. View