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Effects of Sonication on the in Vitro Digestibility and Structural Properties of Buckwheat Protein Isolates

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Specialty Radiology
Date 2020 Sep 24
PMID 32971393
Citations 21
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Abstract

The present work investigated the effects of sonication at different amplitudes and durations on the in vitro digestibility of buckwheat protein isolates (BPIs). The conformation, particle size and microstructures of the BPIs were also studied to explicate the possible mechanisms of the sonication-induced changes. The results showed that sonication conditions of 20 kHz, pulsed on-time 10 s, off-time 5 s, amplitude of 60% and duration of 10 min (SA6T10) improved the digestibility of BPIs from 41.4% (control) to 58.2%. The tertiary structure analysis showed that sonication exposed the hydrophobic core buried inside the protein molecules and broke the intramolecular crosslinks, based on the increase in the surface hydrophobicity and intrinsic fluorescence and the decrease in the disulphide content. The secondary structure analysis showed that SA6T10 decreased the content of β-turn and β-sheet by 40.9% and 22.4%, respectively, and increased the content of anti-parallel β-sheet, random coil, and α-helix by 40.9%, 30.6%, and 25.5%, respectively. The particle size of the control BPIs (427.7 ± 76.7 nm) increased to 2130.8 ± 356.2 nm in the SA6T10 sonicated sample with a corresponding decrease in the polydispersity index from 0.97 ± 0.04 to 0.51 ± 0.13. Moreover, scanning electron microscopy indicated that sonication broke the macroparticles into smaller fragments and changed the surface state of the proteins. Taken together, sonication has proven to be a promising approach for improving the digestibility of buckwheat proteins, which can be explored as a source of plant-based alternative protein for food applications.

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References
1.
Gulseren I, Guzey D, Bruce B, Weiss J . Structural and functional changes in ultrasonicated bovine serum albumin solutions. Ultrason Sonochem. 2006; 14(2):173-83. DOI: 10.1016/j.ultsonch.2005.07.006. View

2.
Secundo F, Guerrieri N . ATR-FT/IR study on the interactions between gliadins and dextrin and their effects on protein secondary structure. J Agric Food Chem. 2005; 53(5):1757-64. DOI: 10.1021/jf049061x. View

3.
Choi S, Ma C . Conformational study of globulin from common buckwheat (Fagopyrum esculentum Moench) by Fourier transform infrared spectroscopy and differential scanning calorimetry. J Agric Food Chem. 2005; 53(20):8046-53. DOI: 10.1021/jf051040v. View

4.
Tomotake H, Shimaoka I, Kayashita J, YOKOYAMA F, Nakajoh M, Kato N . A buckwheat protein product suppresses gallstone formation and plasma cholesterol more strongly than soy protein isolate in hamsters. J Nutr. 2000; 130(7):1670-4. DOI: 10.1093/jn/130.7.1670. View

5.
Guo X, Zhu K, Zhang H, Yao H . Purification and characterization of the antitumor protein from Chinese tartary buckwheat (Fagopyrum tataricum Gaertn.) water-soluble extracts. J Agric Food Chem. 2007; 55(17):6958-61. DOI: 10.1021/jf071032+. View