The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of Silver Nanoparticles Against

Overview

Journal Biomater Investig Dent

Date 2020 Sep 17

PMID 32939454

Citations 120

Authors

Prashik Parvekar

Jayant Palaskar

Sandeep Metgud

Rahul Maria

Smita Dutta

Affiliations

Soon will be listed here.

Abstract

Aim: To determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of silver nanoparticles against . The antimicrobial efficacy of the silver nanoparticles was determined by the standard methods of Clinical and Laboratory Standards Institute (CLSI). Different concentrations of silver nanoparticles were prepared, and MIC was calculated by tube macro-dilution method. The MBC was determined by the lowest concentration that kills 99.9% of the initial bacterial population. The data were analyzed by ANOVA and Tukey's test using SPSS software.

Results: The MIC and MBC of silver nanoparticles against was found to be 0.625 mg/ml.

Conclusion: The result obtained from this study shows that silver nanoparticles have potential bactericidal effects against at a concentration of 0.625 mg/ml. Silver nanoparticles can be incorporated in the root canal medicaments, sealers and irrigants as it possess potent antimicrobial efficacy against .

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References

Correa J, Mori M, Sanches H, da Cruz A, Poiate Jr E, Pola Poiate I . Silver nanoparticles in dental biomaterials. Int J Biomater. 2015; 2015:485275. PMC: 4312639. DOI: 10.1155/2015/485275. View

Zhou Y, Kong Y, Kundu S, Cirillo J, Liang H . Antibacterial activities of gold and silver nanoparticles against Escherichia coli and bacillus Calmette-Guérin. J Nanobiotechnology. 2012; 10:19. PMC: 3405418. DOI: 10.1186/1477-3155-10-19. View

Barros C, Fulaz S, Stanisic D, Tasic L . Biogenic Nanosilver against Multidrug-Resistant Bacteria (MDRB). Antibiotics (Basel). 2018; 7(3). PMC: 6163489. DOI: 10.3390/antibiotics7030069. View

Elechiguerra J, Burt J, Morones J, Camacho-Bragado A, Gao X, Lara H . Interaction of silver nanoparticles with HIV-1. J Nanobiotechnology. 2005; 3:6. PMC: 1190212. DOI: 10.1186/1477-3155-3-6. View

Pinheiro E, Gomes B, Ferraz C, Sousa E, Teixeira F, Souza-Filho F . Microorganisms from canals of root-filled teeth with periapical lesions. Int Endod J. 2003; 36(1):1-11. DOI: 10.1046/j.1365-2591.2003.00603.x. View

Feng Q, Wu J, Chen G, Cui F, Kim T, Kim J . A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus. J Biomed Mater Res. 2000; 52(4):662-8. DOI: 10.1002/1097-4636(20001215)52:4<662::aid-jbm10>3.0.co;2-3. View

Prasher P, Singh M, Mudila H . Silver nanoparticles as antimicrobial therapeutics: current perspectives and future challenges. 3 Biotech. 2018; 8(10):411. PMC: 6138003. DOI: 10.1007/s13205-018-1436-3. View

McCormack M, Smith A, Akram A, Jackson M, Robertson D, Edwards G . Staphylococcus aureus and the oral cavity: an overlooked source of carriage and infection?. Am J Infect Control. 2015; 43(1):35-7. DOI: 10.1016/j.ajic.2014.09.015. View

Estrela C, Rodrigues de Araujo Estrela C, Bammann L, Pecora J . Two methods to evaluate the antimicrobial action of calcium hydroxide paste. J Endod. 2002; 27(12):720-3. DOI: 10.1097/00004770-200112000-00002. View

10.

Hosny A, Rasmy S, Aboul-Magd D, Kashef M, El-Bazza Z . The increasing threat of silver-resistance in clinical isolates from wounds and burns. Infect Drug Resist. 2019; 12:1985-2001. PMC: 6636436. DOI: 10.2147/IDR.S209881. View

11.

Jhajharia K, Parolia A, Shetty K, Mehta L . Biofilm in endodontics: A review. J Int Soc Prev Community Dent. 2015; 5(1):1-12. PMC: 4355843. DOI: 10.4103/2231-0762.151956. View

12.

Li Y, Leung P, Yao L, Song Q, Newton E . Antimicrobial effect of surgical masks coated with nanoparticles. J Hosp Infect. 2005; 62(1):58-63. DOI: 10.1016/j.jhin.2005.04.015. View

13.

Matsumura Y, Yoshikata K, Kunisaki S, Tsuchido T . Mode of bactericidal action of silver zeolite and its comparison with that of silver nitrate. Appl Environ Microbiol. 2003; 69(7):4278-81. PMC: 165194. DOI: 10.1128/AEM.69.7.4278-4281.2003. View

14.

Morones J, Elechiguerra J, Camacho A, Holt K, Kouri J, Tapia Ramirez J . The bactericidal effect of silver nanoparticles. Nanotechnology. 2010; 16(10):2346-53. DOI: 10.1088/0957-4484/16/10/059. View

15.

Chen X, Schluesener H . Nanosilver: a nanoproduct in medical application. Toxicol Lett. 2007; 176(1):1-12. DOI: 10.1016/j.toxlet.2007.10.004. View

16.

Yang H, Jun H, Jung Y, Choi B . Enterococcus faecalis activates caspase-1 leading to increased interleukin-1 beta secretion in macrophages. J Endod. 2014; 40(10):1587-92. DOI: 10.1016/j.joen.2014.06.015. View

17.

Lara H, Garza-Trevino E, Ixtepan-Turrent L, Singh D . Silver nanoparticles are broad-spectrum bactericidal and virucidal compounds. J Nanobiotechnology. 2011; 9:30. PMC: 3199605. DOI: 10.1186/1477-3155-9-30. View

18.

Shweta , Prakash S . Dental abscess: A microbiological review. Dent Res J (Isfahan). 2013; 10(5):585-91. PMC: 3858730. View

19.

Lok C, Ho C, Chen R, He Q, Yu W, Sun H . Silver nanoparticles: partial oxidation and antibacterial activities. J Biol Inorg Chem. 2007; 12(4):527-34. DOI: 10.1007/s00775-007-0208-z. View

20.

Velazquez-Meza M . [Staphylococcus aureus methicillin-resistant: emergence and dissemination]. Salud Publica Mex. 2005; 47(5):381-7. DOI: 10.1590/s0036-36342005000500009. View