Mass Spectrometry and Structural Biology Techniques in the Studies on the Coronavirus-Receptor Interaction

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2020 Sep 15

PMID 32927621

Citations 7

Authors

Danuta Witkowska

Affiliations

Soon will be listed here.

Abstract

Mass spectrometry and some other biophysical methods, have made substantial contributions to the studies on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and human proteins interactions. The most interesting feature of SARS-CoV-2 seems to be the structure of its spike (S) protein and its interaction with the human cell receptor. Mass spectrometry of spike S protein revealed how the glycoforms are distributed across the S protein surface. X-ray crystallography and cryo-electron microscopy made huge impact on the studies on the S protein and ACE2 receptor protein interaction, by elucidating the three-dimensional structures of these proteins and their conformational changes. The findings of the most recent studies in the scope of SARS-CoV-2-Human protein-protein interactions are described here.

Citing Articles

Fluorescence, Circular Dichroism and Mass Spectrometry as Tools to Study Virus Structure.

Neira J Subcell Biochem. 2024; 105:207-245.

PMID: 39738948 DOI: 10.1007/978-3-031-65187-8_6.

Analytical Ultracentrifugation Detects Quaternary Rearrangements and Antibody-Induced Conformational Selection of the SARS-CoV-2 Spike Trimer.

Guerrini G, Mehn D, Fumagalli F, Gioria S, Pedotti M, Simonelli L Int J Mol Sci. 2023; 24(19).

PMID: 37834322 PMC: 10573103. DOI: 10.3390/ijms241914875.

Thermodynamic analysis of the interactions between human ACE2 and spike RBD of Betacoronaviruses (SARS-CoV-1 and SARS-CoV-2).

Rombel-Bryzek A, Miller A, Witkowska D FEBS Open Bio. 2022; 13(1):174-184.

PMID: 36416453 PMC: 9808565. DOI: 10.1002/2211-5463.13525.

Site-specific glycosylation of SARS-CoV-2: Big challenges in mass spectrometry analysis.

Campos D, Girgis M, Sanda M Proteomics. 2022; 22(15-16):e2100322.

PMID: 35700310 PMC: 9349404. DOI: 10.1002/pmic.202100322.

Perspectives and potential approaches for targeting neuropilin 1 in SARS-CoV-2 infection.

Chapoval S, Keegan A Mol Med. 2021; 27(1):162.

PMID: 34961486 PMC: 8711287. DOI: 10.1186/s10020-021-00423-y.

References

Weiskopf D, Schmitz K, Raadsen M, Grifoni A, Okba N, Endeman H . Phenotype and kinetics of SARS-CoV-2-specific T cells in COVID-19 patients with acute respiratory distress syndrome. Sci Immunol. 2020; 5(48). PMC: 7319493. DOI: 10.1126/sciimmunol.abd2071. View

Belouzard S, Chu V, Whittaker G . Activation of the SARS coronavirus spike protein via sequential proteolytic cleavage at two distinct sites. Proc Natl Acad Sci U S A. 2009; 106(14):5871-6. PMC: 2660061. DOI: 10.1073/pnas.0809524106. View

Sikkema R, Farag E, Himatt S, Ibrahim A, Al-Romaihi H, Al-Marri S . Risk Factors for Primary Middle East Respiratory Syndrome Coronavirus Infection in Camel Workers in Qatar During 2013-2014: A Case-Control Study. J Infect Dis. 2017; 215(11):1702-1705. PMC: 7107360. DOI: 10.1093/infdis/jix174. View

Koo J, Cook A, Park M, Sun Y, Sun H, Lim J . Interventions to mitigate early spread of SARS-CoV-2 in Singapore: a modelling study. Lancet Infect Dis. 2020; 20(6):678-688. PMC: 7158571. DOI: 10.1016/S1473-3099(20)30162-6. View

Tian X, Li C, Huang A, Xia S, Lu S, Shi Z . Potent binding of 2019 novel coronavirus spike protein by a SARS coronavirus-specific human monoclonal antibody. Emerg Microbes Infect. 2020; 9(1):382-385. PMC: 7048180. DOI: 10.1080/22221751.2020.1729069. View

Chan J, Yuan S, Kok K, To K, Chu H, Yang J . A familial cluster of pneumonia associated with the 2019 novel coronavirus indicating person-to-person transmission: a study of a family cluster. Lancet. 2020; 395(10223):514-523. PMC: 7159286. DOI: 10.1016/S0140-6736(20)30154-9. View

Struwe W, Emmott E, Bailey M, Sharon M, Sinz A, Corrales F . The COVID-19 MS Coalition-accelerating diagnostics, prognostics, and treatment. Lancet. 2020; 395(10239):1761-1762. PMC: 7255197. DOI: 10.1016/S0140-6736(20)31211-3. View

Zhou P, Yang X, Wang X, Hu B, Zhang L, Zhang W . A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020; 579(7798):270-273. PMC: 7095418. DOI: 10.1038/s41586-020-2012-7. View

Wrapp D, Wang N, Corbett K, Goldsmith J, Hsieh C, Abiona O . Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation. Science. 2020; 367(6483):1260-1263. PMC: 7164637. DOI: 10.1126/science.abb2507. View

10.

Reusken C, Haagmans B, Muller M, Gutierrez C, Godeke G, Meyer B . Middle East respiratory syndrome coronavirus neutralising serum antibodies in dromedary camels: a comparative serological study. Lancet Infect Dis. 2013; 13(10):859-66. PMC: 7106530. DOI: 10.1016/S1473-3099(13)70164-6. View

11.

Tilocca B, Soggiu A, Sanguinetti M, Musella V, Britti D, Bonizzi L . Comparative computational analysis of SARS-CoV-2 nucleocapsid protein epitopes in taxonomically related coronaviruses. Microbes Infect. 2020; 22(4-5):188-194. PMC: 7156246. DOI: 10.1016/j.micinf.2020.04.002. View

12.

Hilgenfeld R . From SARS to MERS: crystallographic studies on coronaviral proteases enable antiviral drug design. FEBS J. 2014; 281(18):4085-96. PMC: 7163996. DOI: 10.1111/febs.12936. View

13.

Lu R, Zhao X, Li J, Niu P, Yang B, Wu H . Genomic characterisation and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding. Lancet. 2020; 395(10224):565-574. PMC: 7159086. DOI: 10.1016/S0140-6736(20)30251-8. View

14.

Ksiazek T, Erdman D, Goldsmith C, Zaki S, Peret T, Emery S . A novel coronavirus associated with severe acute respiratory syndrome. N Engl J Med. 2003; 348(20):1953-66. DOI: 10.1056/NEJMoa030781. View

15.

Prajapat M, Sarma P, Shekhar N, Avti P, Sinha S, Kaur H . Drug targets for corona virus: A systematic review. Indian J Pharmacol. 2020; 52(1):56-65. PMC: 7074424. DOI: 10.4103/ijp.IJP_115_20. View

16.

Delamater P, Street E, Leslie T, Yang Y, Jacobsen K . Complexity of the Basic Reproduction Number (R). Emerg Infect Dis. 2018; 25(1):1-4. PMC: 6302597. DOI: 10.3201/eid2501.171901. View

17.

Tang T, Bidon M, Jaimes J, Whittaker G, Daniel S . Coronavirus membrane fusion mechanism offers a potential target for antiviral development. Antiviral Res. 2020; 178:104792. PMC: 7194977. DOI: 10.1016/j.antiviral.2020.104792. View

18.

Li Q, Guan X, Wu P, Wang X, Zhou L, Tong Y . Early Transmission Dynamics in Wuhan, China, of Novel Coronavirus-Infected Pneumonia. N Engl J Med. 2020; 382(13):1199-1207. PMC: 7121484. DOI: 10.1056/NEJMoa2001316. View

19.

Follis K, York J, Nunberg J . Furin cleavage of the SARS coronavirus spike glycoprotein enhances cell-cell fusion but does not affect virion entry. Virology. 2006; 350(2):358-69. PMC: 7111780. DOI: 10.1016/j.virol.2006.02.003. View

20.

Wang C, Horby P, Hayden F, Gao G . A novel coronavirus outbreak of global health concern. Lancet. 2020; 395(10223):470-473. PMC: 7135038. DOI: 10.1016/S0140-6736(20)30185-9. View