Sp. Nov., Isolated from Pine Grove Soil, Exhibiting Antimicrobial Potential

A polyphasic study was carried out to establish the taxonomic position of an acidophilic isolate designated MMS16-CNU292 (=JCM 32302) from pine grove soil, and provisionally assigned to the genus . On the basis of 16S rRNA gene sequence similarity, the strain formed a novel evolutionary lineage within and showed highest similarities to KCTC 9699 (98.75 %), IFO 15206 (98.74 %), NRRL B-1817 (98.61 %) and HKI 0315 (98.42 %), respectively. Strain MMS16-CNU292 possessed MK-9(H) and MK-9(H) as the major menaquinones, and a major amount of -diaminopimelic acid in the cell-wall peptidoglycan. The whole-cell hydrolysates were rich in galactose, glucose and mannose, and the polar lipids mainly consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol and phosphatidylinositol mannosides. The major fatty acids were anteiso-C-A, anteiso-C, and iso-C, and the DNA G+C content was 71.5 mol%. The strain exhibited antibacterial activity against a number of bacterial strains, and the activity was generally greater when grown in acidic conditions. The phylogenetic, chemotaxonomic and phenotypic properties enabled distinction of MMS16-CNU292 from related species, and thus the isolate should be recognized as a new species of the genus , for which the name sp. nov. (type strain=MMS16-CNU292=KCTC 49011=JCM 32302) is proposed.