Induced Pluripotent Stem Cell-derived Vascular Smooth Muscle Cells

Overview

Journal Vasc Biol

Date 2020 Sep 14

PMID 32923972

Citations 8

Authors

Makeda Stephenson

Daniel H Reich

Kenneth R Boheler

Affiliations

Soon will be listed here.

Abstract

The reproducible generation of human-induced pluripotent stem cell (hiPSC)-derived vascular smooth muscle cells (vSMCs) has been critical to overcoming many limitations of animal and primary cell models of vascular biology and disease. Since this initial advance, research in the field has turned toward recapitulating the naturally occurring subtype specificity found in vSMCs throughout the body, and honing functional models of vascular disease. In this review, we summarize vSMC derivation approaches, including current phenotype and developmental origin-specific methods, and applications of vSMCs in functional disease models and engineered tissues. Further, we discuss the challenges of heterogeneity in hiPSC-derived tissues and propose approaches to identify and isolate vSMC subtype populations.

Citing Articles

Dynamic three dimensional environment for efficient and large scale generation of smooth muscle cells from hiPSCs.

Green A, Wei Y, Warram J, Hartman Y, Geng X, Nguyen T Stem Cell Res Ther. 2024; 15(1):463.

PMID: 39627821 PMC: 11616106. DOI: 10.1186/s13287-024-04053-z.

Use of iPSC-Derived Smooth Muscle Cells to Model Physiology and Pathology.

Kwartler C, Pinelo J Arterioscler Thromb Vasc Biol. 2024; 44(7):1523-1536.

PMID: 38695171 PMC: 11209779. DOI: 10.1161/ATVBAHA.123.319703.

The challenges and prospects of smooth muscle tissue engineering.

Baldwin C, Iyer S, Rao R Regen Med. 2024; 19(3):135-143.

PMID: 38440898 PMC: 10941056. DOI: 10.2217/rme-2023-0230.

Vascular organs-on-chip made with patient-derived endothelial cells: technologies to transform drug discovery and disease modeling.

Whitworth C, Polacheck W Expert Opin Drug Discov. 2023; 19(3):339-351.

PMID: 38117223 PMC: 10922379. DOI: 10.1080/17460441.2023.2294947.

Induced pluripotent stem cell-derived smooth muscle cells to study cardiovascular calcification.

Atkins S, Sonawane A, Brouwhuis R, Barrientos J, Ha A, Rogers M Front Cardiovasc Med. 2022; 9:925777.

PMID: 35958427 PMC: 9357895. DOI: 10.3389/fcvm.2022.925777.

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