Effect of Overexpression of on Intracellular K/Na Balance in Transgenic Poplar ( × ) Under Salt Stress

Salt stress is one of the main factors that affect both growth and development of plants. Maintaining K/Na balance in the cytoplasm is important for metabolism as well as salt resistance in plants. In the present study, we monitored the growth (height and diameter) of transgenic × trees (ABJ01) carrying gene (a tomato jasmonic/ethylene responsive factors gene) over 4 years, which showed faster growth and significant salt tolerance compared with non-transgenic poplar trees (9#). The expression of and genes that encode Na/H antiporters in the vacuole and plasma membranes was measured in leaves under NaCl stress. Non-invasive micro-test techniques (NMT) were used to analyse ion flux of Na, K, and H in the root tip of seedlings under treatment with100 mM NaCl for 7, 15, and 30 days. Results showed that the expression of and was much higher in ABJ01 compared with 9#, and the Na efflux and H influx fluxes of root were remarkable higher in ABJ01 than in 9#, but K efflux exhibited lower level. All above suggest that salt stress induces and to a greater expression level in ABJ01, resulting in the accumulation of Na/H antiporter to better maintain K/Na balance in the cytoplasm of this enhanced salt resistant variety. This may help us to better understand the mechanism of transgenic poplars with improving salt tolerance by overexpressing and could provide a basis for future breeding programs aimed at improving salt resistance in transgenic poplar.