Rapid and Quantitative Detection of SARS-CoV-2 Specific IgG for Convalescent Serum Evaluation

Overview

Journal Biosens Bioelectron

Specialties Biochemistry
Biotechnology

Date 2020 Sep 11

PMID 32916610

Citations 39

Authors

Xiaotian Tan

Mila Krel

Enriko Dolgov

Steven Park

Xuzhou Li

Weishu Wu

Yun-Lu Sun

Jie Zhang

Maung Kyaw Khaing Oo

David S Perlin

Xudong Fan

Affiliations

Soon will be listed here.

Abstract

Convalescent serum with a high abundance of neutralization IgG is a promising therapeutic agent for rescuing COVID-19 patients in the critical stage. Knowing the concentration of SARS-CoV-2 S1-specific IgG is crucial in selecting appropriate convalescent serum donors. Here, we present a portable microfluidic ELISA technology for rapid (15 min), quantitative, and sensitive detection of anti-SARS-CoV-2 S1 IgG in human serum with only 8 μL sample volume. We first identified a humanized monoclonal IgG that has a high binding affinity and a relatively high specificity towards SARS-CoV-2 S1 protein, which can subsequently serve as the calibration standard of anti-SARS-CoV-2 S1 IgG in serological analyses. We then measured the abundance of anti-SARS-CoV-2 S1 IgG in 16 convalescent COVID-19 patients. Due to the availability of the calibration standard and the large dynamic range of our assay, we were able to identify "qualified donors" for convalescent serum therapy with only one fixed dilution factor (200 ×). Finally, we demonstrated that our technology can sensitively detect SARS-CoV-2 antigens (S1 and N proteins) with pg/mL level sensitivities in 40 min. Overall, our technology can greatly facilitate rapid, sensitive, and quantitative analysis of COVID-19 related markers for therapeutic, diagnostic, epidemiologic, and prognostic purposes.

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