Toxoplasma Invasion Delayed by TgERK7 Eradication

Overview

Journal Parasitol Res

Specialty Parasitology

Date 2020 Sep 11

PMID 32914221

Authors

Zhong-Yuan Li

Xue Liang

Hai-Ting Guo

Jie Tan

Xing-Quan Zhu

Quan Liu

Affiliations

Soon will be listed here.

Abstract

Toxoplasma gondii causes serious clinical toxoplasmosis in humans mostly due to its asexual life cycles, which can be artificially divided into five tightly coterminous stages. Any radical or delay for the stage will result in tremendous changes immediately behind. We previously demonstrated that TgERK7 is associated with the intracellular proliferation of T. gondii, but during the process, other stages before were not meanwhile determined. To further clarify the function of ERK7 gene in T. gondii, the complemental strain of ΔTgERK7 tachyzoites created previously was engineered via electric transfection with the recombinant pUC/Tgerk7 plasmid, named pUC/TgERK7 strain in this study, and was used together with ΔTgERK7 and wild-type GT1 strains to retrospect the phenotypic changes including invasion and attachment. The results showed that TgERK7 protein can be re-expressed in the ΔTgERK7 tachyzoites and eradication of this protein leads to significantly lower invasion of T. gondii at 1 h and 2 h post-infection (P < 0.05), which is the key factor causing the following slow intracellular proliferation, in comparison with wild-type GT1 and pUC/TgERK7 parasites; noteworthily, at other early time points including 15 min for attachment assay was no statistical difference (P > 0.05). The data suggested that ERK7 protein in T. gondii is an important virulence factor that participates in the invasion of this parasite.

References

An R, Tang Y, Chen L, Cai H, Lai D, Liu K . Encephalitis is mediated by ROP18 of , a severe pathogen in AIDS patients. Proc Natl Acad Sci U S A. 2018; 115(23):E5344-E5352. PMC: 6003310. DOI: 10.1073/pnas.1801118115. View

Blader I, Coleman B, Chen C, Gubbels M . Lytic Cycle of Toxoplasma gondii: 15 Years Later. Annu Rev Microbiol. 2015; 69:463-85. PMC: 4659696. DOI: 10.1146/annurev-micro-091014-104100. View

Dard C, Marty P, Brenier-Pinchart M, Garnaud C, Fricker-Hidalgo H, Pelloux H . Management of toxoplasmosis in transplant recipients: an update. Expert Rev Anti Infect Ther. 2018; 16(6):447-460. DOI: 10.1080/14787210.2018.1483721. View

Ghosh S, Chisholm S, Dans M, Lakkavaram A, Kennedy K, Ralph S . The cysteine protease dipeptidyl aminopeptidase 3 does not contribute to egress of Plasmodium falciparum from host red blood cells. PLoS One. 2018; 13(3):e0193538. PMC: 5839547. DOI: 10.1371/journal.pone.0193538. View

Guo H, Li Z, Wang J, Geng Z, Zhu X . Immune Responses Induced by pVAX/ERK7 against Infection in BALB/c Mice. Iran J Parasitol. 2020; 14(4):552-562. PMC: 7028233. View

Hehl A, Basso W, Lippuner C, Ramakrishnan C, Okoniewski M, Walker R . Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes. BMC Genomics. 2015; 16:66. PMC: 4340605. DOI: 10.1186/s12864-015-1225-x. View

Kim K, Weiss L . Toxoplasma gondii: the model apicomplexan. Int J Parasitol. 2004; 34(3):423-32. PMC: 3086386. DOI: 10.1016/j.ijpara.2003.12.009. View

Kim K, Soldati D, Boothroyd J . Gene replacement in Toxoplasma gondii with chloramphenicol acetyltransferase as selectable marker. Science. 1993; 262(5135):911-4. DOI: 10.1126/science.8235614. View

Li Z, Wang Z, Huang S, Zhu X, Liu Q . TgERK7 is involved in the intracellular proliferation of Toxoplasma gondii. Parasitol Res. 2016; 115(9):3419-24. DOI: 10.1007/s00436-016-5103-5. View

10.

Li Z, Guo H, Calderon-Mantilla G, He J, Wang J, Bonev B . Immunostimulatory efficacy and protective potential of putative TgERK7 protein in mice experimentally infected by Toxoplasma gondii. Int J Med Microbiol. 2020; 310(5):151432. DOI: 10.1016/j.ijmm.2020.151432. View

11.

Li Z, Guo H, Tan J, Geng Z, Zhu X . Devitalization of the immune mapped protein 1 undermines the intracellular proliferation of Toxoplasma gondii. Exp Parasitol. 2020; 211:107843. DOI: 10.1016/j.exppara.2020.107843. View

12.

Messina M, Niesman I, Mercier C, Sibley L . Stable DNA transformation of Toxoplasma gondii using phleomycin selection. Gene. 1995; 165(2):213-7. DOI: 10.1016/0378-1119(95)00548-k. View

13.

Omata Y, Taka A, Terada K, Koyama T, Kanda M, Saito A . Isolation of coccidian enteroepithelial stages of Toxoplasma gondii from the intestinal mucosa of cats by Percoll density-gradient centrifugation. Parasitol Res. 1997; 83(6):574-7. DOI: 10.1007/s004360050300. View

14.

Pittman K, Knoll L . Long-Term Relationships: the Complicated Interplay between the Host and the Developmental Stages of Toxoplasma gondii during Acute and Chronic Infections. Microbiol Mol Biol Rev. 2015; 79(4):387-401. PMC: 4557073. DOI: 10.1128/MMBR.00027-15. View

15.

Shen B, Brown K, Lee T, Sibley L . Efficient gene disruption in diverse strains of Toxoplasma gondii using CRISPR/CAS9. mBio. 2014; 5(3):e01114-14. PMC: 4030483. DOI: 10.1128/mBio.01114-14. View

16.

Sibley L, Messina M, Niesman I . Stable DNA transformation in the obligate intracellular parasite Toxoplasma gondii by complementation of tryptophan auxotrophy. Proc Natl Acad Sci U S A. 1994; 91(12):5508-12. PMC: 44025. DOI: 10.1073/pnas.91.12.5508. View

17.

Smith J . Tracking transmission of the zoonosis Toxoplasma gondii. Adv Parasitol. 2009; 68:139-59. DOI: 10.1016/S0065-308X(08)00606-4. View

18.

Sun H, Zhuo X, Zhao X, Yang Y, Chen X, Yao C . The heat shock protein 90 of Toxoplasma gondii is essential for invasion of host cells and tachyzoite growth. Parasite. 2017; 24:22. PMC: 5479401. DOI: 10.1051/parasite/2017023. View

19.

Szabo E, Finney C . Toxoplasma gondii: One Organism, Multiple Models. Trends Parasitol. 2016; 33(2):113-127. DOI: 10.1016/j.pt.2016.11.007. View

20.

Thabet A, Schmidt J, Baumann S, Honscha W, von Bergen M, Daugschies A . Resistance towards monensin is proposed to be acquired in a Toxoplasma gondii model by reduced invasion and egress activities, in addition to increased intracellular replication. Parasitology. 2017; 145(3):313-325. DOI: 10.1017/S0031182017001512. View