The Heat Shock Protein 90 of Toxoplasma Gondii is Essential for Invasion of Host Cells and Tachyzoite Growth

Overview

Journal Parasite

Specialty Parasitology

Date 2017 Jun 20

PMID 28627357

Citations 18

Authors

Hongchao Sun

Xunhui Zhuo

Xianfeng Zhao

Yi Yang

Xueqiu Chen

Chaoqun Yao

Aifang Du

Affiliations

Soon will be listed here.

Abstract

Toxoplasma gondii is an obligate intracellular apicomplexan parasite that infects almost all warm-blooded vertebrates. Heat shock proteins (HSP) regulate key signal transduction events in many organisms, and heat shock protein 90 (Hsp90) plays an important role in growth, development, and virulence in several parasitic protozoa. Here, we discovered increased transcription of the Hsp90 gene under conditions for bradyzoite differentiation, i.e. alkaline and heat shock conditions in vitro, suggesting that Hsp90 may be connected with bradyzoite development in T. gondii. A knockout of the TgHsp90 strain (ΔHsp90) and a complementation strain were constructed. The TgHsp90 knockout cells were found to be defective in host-cell invasion, were not able to proliferate in vitro in Vero cells, and did not show long-time survival in mice in vivo. These inabilities of the knockout parasites were restored upon complementation of TgHsp90. These data unequivocally show that TgHsp90 contributes to bradyzoite development, and to invasion and replication of T. gondii in host cells.

Citing Articles

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Toxoplasma gondii bradyzoite-specific BAG1 is nonessential for cyst formation due to compensation by other heat-shock proteins.

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Is the -Terminal Domain an Effective and Selective Target for the Design of Hsp90 Inhibitors against Yeast?.

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The Role of Some Free-Ranging Animals in the Transmission of Multi-Host Species of Spp.

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