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Elevated Levels of Autoantibodies Against DNAJC2 in Sera of Patients with Atherosclerotic Diseases

Overview

Overview

Journal Heliyon

Specialty Social Sciences

Date 2020 Sep 9

PMID 32904265

Citations 13

Authors

Authors

Yoichi Yoshida

Xiao-Meng Zhang

Hao Wang

Toshio Machida

Seiichiro Mine

Eiichi Kobayashi

Akihiko Adachi

Tomoo Matsutani

Ikuo Kamitsukasa

Takeshi Wada

Akiyo Aotsuka

Katsuro Iwase

Go Tomiyoshi

Rika Nakamura

Natsuko Shinmen

Hideyuki Kuroda

Hirotaka Takizawa

Koichi Kashiwado

Hideo Shin

Yuichi Akaogi

Junichiro Shimada

Eiichiro Nishi

Mikiko Ohno

Minoru Takemoto

Koutaro Yokote

Kenichiro Kitamura

Yasuo Iwadate

Takaki Hiwasa

Affiliations

Affiliations

Soon will be listed here.

Abstract

Background: Serum antibody markers have been increasingly identified not only for cancer and autoimmune diseases but also for atherosclerosis-related diseases such as acute ischemic stroke (AIS), acute myocardial infarction (AMI), diabetes mellitus (DM), and chronic kidney disease (CKD). Biomarkers for transient ischemic attack (TIA) and non-ST segment elevation acute coronary syndrome (NSTEACS) are potentially useful for detection of early phase of atherosclerotic changes against AIS and AMI, respectively.

Methods: We utilized serological identification of antigens by recombinant cDNA expression cloning (SEREX) using a human aortic endothelial cell cDNA phage library and sera from patients with TIA or NSTEACS. Serum antibody levels were measured by amplified luminescent proximity homogeneous assay-linked immunosorbent assay (AlphaLISA) using purified recombinant antigens.

Results: Screening of sera from patients with TIA identified DnaJ heat shock protein family (Hsp40) member C2 (DNAJC2) as a candidate antigen, which was also isolated by SEREX screening using sera of patients with NSTEACS. The validation cohort revealed significantly higher DNAJC2 antibody (DNAJC2-Ab) levels in the sera of patients with TIA or AIS than those in healthy donors (HDs). Multivariate logistic regression analysis indicated that the predictive odds ratios (OR) of DNAJC2-Ab levels for TIA and AIS were 2.54 (95% confidence interval [CI]: 1.36-4.74, = 0.0034) and 2.14 (95% CI: 1.39-3.30, = 0.0005), respectively. Serum DNAJC2-Ab levels were also higher in patients with AMI, DM, and CKD than those in HDs.

Conclusion: Serum DNAJC2-Ab level may be useful for early detection of atherosclerotic lesions, which lead to AIS and AMI.

Citing Articles

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Yamagata H, Hayashi A, Yoshida Y, Koshizaka M, Onishi S, Yoshida T Sci Rep. 2023; 13(1):5391.

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Utility of atherosclerosis-associated serum antibodies against colony-stimulating factor 2 in predicting the onset of acute ischemic stroke and prognosis of colorectal cancer.

Li S, Yoshida Y, Kubota M, Zhang B, Matsutani T, Ito M Front Cardiovasc Med. 2023; 10:1042272.

PMID: 36844744 PMC: 9954151. DOI: 10.3389/fcvm.2023.1042272.

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