BZIP Dimers CREB1, ATF2, Zta, ATF3|cJun, and CFos|cJun Prefer to Bind to Some Double-Stranded DNA Sequences Containing 5-Formylcytosine and 5-Carboxylcytosine

Overview

Journal Biochemistry

Specialty Biochemistry

Date 2020 Sep 9

PMID 32902247

Citations 1

Authors

Sreejana Ray

Desiree Tillo

Aniekanabasi Ufot

Nima Assad

Stewart Durell

Charles Vinson

Affiliations

Soon will be listed here.

Abstract

In mammalian cells, 5-methylcytosine (5mC) occurs in genomic double-stranded DNA (dsDNA) and is enzymatically oxidized to 5-hydroxymethylcytosine (5hmC), then to 5-formylcytosine (5fC), and finally to 5-carboxylcytosine (5caC). These cytosine modifications are enriched in regulatory regions of the genome. The effect of these oxidative products on five bZIP dimers (CREB1, ATF2, Zta, ATF3|cJun, and cFos|cJun) binding to five types of dsDNA was measured using protein binding microarrays. The five dsDNAs contain either cytosine in both DNA strands or cytosine in one strand and either 5mC, 5hmC, 5fC, or 5caC in the second strand. Some sequences containing the CEBP half-site GCAA are bound more strongly by all five bZIP domains when dsDNA contains 5mC, 5hmC, or 5fC. dsDNA containing 5caC in some TRE (AP-1)-like sequences, e.g., TGACTAA, is better bound by Zta, ATF3|cJun, and cFos|cJun.

Citing Articles

Altering the Double-Stranded DNA Specificity of the bZIP Domain of Zta with Site-Directed Mutagenesis at N182.

Ray S, Tillo D, Assad N, Ufot A, Porollo A, Durell S ACS Omega. 2022; 7(1):129-139.

PMID: 35036684 PMC: 8756438. DOI: 10.1021/acsomega.1c04148.

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