Exoproteomics for Better Understanding Virulence

Overview

Journal Toxins (Basel)

Publisher MDPI

Specialty Toxicology

Date 2020 Sep 9

PMID 32899849

Citations 12

Authors

Salome Sauvage

Julie Hardouin

Affiliations

Soon will be listed here.

Abstract

is the most common human opportunistic pathogen associated with nosocomial diseases. In 2017, the World Health Organization has classified as a critical agent threatening human health, and for which the development of new treatments is urgently necessary. One interesting avenue is to target virulence factors to understand pathogenicity. Thus, characterising exoproteins of is a hot research topic and proteomics is a powerful approach that provides important information to gain insights on bacterial virulence. The aim of this review is to focus on the contribution of proteomics to the studies of exoproteins, highlighting its relevance in the discovery of virulence factors, post-translational modifications on exoproteins and host-pathogen relationships.

Citing Articles

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References

Clamens T, Rosay T, Crepin A, Grandjean T, Kentache T, Hardouin J . The aliphatic amidase AmiE is involved in regulation of Pseudomonas aeruginosa virulence. Sci Rep. 2017; 7:41178. PMC: 5259723. DOI: 10.1038/srep41178. View

Rao P, Yamada Y, Tan Y, Leung K . Use of proteomics to identify novel virulence determinants that are required for Edwardsiella tarda pathogenesis. Mol Microbiol. 2004; 53(2):573-86. DOI: 10.1111/j.1365-2958.2004.04123.x. View

Hickey C, Schaible B, Nguyen S, Hurley D, Srikumar S, Fanning S . Increased Virulence of Bloodstream Over Peripheral Isolates of Identified Through Post-transcriptional Regulation of Virulence Factors. Front Cell Infect Microbiol. 2018; 8:357. PMC: 6212473. DOI: 10.3389/fcimb.2018.00357. View

Suriyanarayanan T, Periasamy S, Lin M, Ishihama Y, Swarup S . Flagellin FliC Phosphorylation Affects Type 2 Protease Secretion and Biofilm Dispersal in Pseudomonas aeruginosa PAO1. PLoS One. 2016; 11(10):e0164155. PMC: 5049796. DOI: 10.1371/journal.pone.0164155. View

Gaviard C, Broutin I, Cosette P, De E, Jouenne T, Hardouin J . Lysine Succinylation and Acetylation in Pseudomonas aeruginosa. J Proteome Res. 2018; 17(7):2449-2459. DOI: 10.1021/acs.jproteome.8b00210. View

Spooner R, Yilmaz O . Nucleoside-diphosphate-kinase: a pleiotropic effector in microbial colonization under interdisciplinary characterization. Microbes Infect. 2011; 14(3):228-37. PMC: 3277739. DOI: 10.1016/j.micinf.2011.10.002. View

Ren J, Sang Y, Tan Y, Tao J, Ni J, Liu S . Acetylation of Lysine 201 Inhibits the DNA-Binding Ability of PhoP to Regulate Salmonella Virulence. PLoS Pathog. 2016; 12(3):e1005458. PMC: 4778762. DOI: 10.1371/journal.ppat.1005458. View

Silverman J, Agnello D, Zheng H, Andrews B, Li M, Catalano C . Haemolysin coregulated protein is an exported receptor and chaperone of type VI secretion substrates. Mol Cell. 2013; 51(5):584-93. PMC: 3844553. DOI: 10.1016/j.molcel.2013.07.025. View

Fulton K, Baltat I, Twine S . Immunoproteomics Methods and Techniques. Methods Mol Biol. 2019; 2024:25-58. DOI: 10.1007/978-1-4939-9597-4_2. View

10.

Llamas M, van der Sar A . Assessing Pseudomonas virulence with nonmammalian host: zebrafish. Methods Mol Biol. 2014; 1149:709-21. DOI: 10.1007/978-1-4939-0473-0_55. View

11.

Hentchel K, Escalante-Semerena J . Acylation of Biomolecules in Prokaryotes: a Widespread Strategy for the Control of Biological Function and Metabolic Stress. Microbiol Mol Biol Rev. 2015; 79(3):321-46. PMC: 4503791. DOI: 10.1128/MMBR.00020-15. View

12.

Scott N, Hare N, White M, Manos J, Cordwell S . Secretome of transmissible Pseudomonas aeruginosa AES-1R grown in a cystic fibrosis lung-like environment. J Proteome Res. 2013; 12(12):5357-69. DOI: 10.1021/pr4007365. View

13.

Mace C, Seyer D, Chemani C, Cosette P, Di-Martino P, Guery B . Identification of biofilm-associated cluster (bac) in Pseudomonas aeruginosa involved in biofilm formation and virulence. PLoS One. 2008; 3(12):e3897. PMC: 2587700. DOI: 10.1371/journal.pone.0003897. View

14.

Koppenol-Raab M, Sjoelund V, Manes N, Gottschalk R, Dutta B, Benet Z . Proteome and Secretome Analysis Reveals Differential Post-transcriptional Regulation of Toll-like Receptor Responses. Mol Cell Proteomics. 2017; 16(4 suppl 1):S172-S186. PMC: 5393387. DOI: 10.1074/mcp.M116.064261. View

15.

Gaviard C, Jouenne T, Hardouin J . Proteomics of Pseudomonas aeruginosa: the increasing role of post-translational modifications. Expert Rev Proteomics. 2018; 15(9):757-772. DOI: 10.1080/14789450.2018.1516550. View

16.

Armengaud J, Christie-Oleza J, Clair G, Malard V, Duport C . Exoproteomics: exploring the world around biological systems. Expert Rev Proteomics. 2012; 9(5):561-75. DOI: 10.1586/epr.12.52. View

17.

Perez-Llarena F, Bou G . Proteomics As a Tool for Studying Bacterial Virulence and Antimicrobial Resistance. Front Microbiol. 2016; 7:410. PMC: 4814472. DOI: 10.3389/fmicb.2016.00410. View

18.

Mougous J, Gifford C, Ramsdell T, Mekalanos J . Threonine phosphorylation post-translationally regulates protein secretion in Pseudomonas aeruginosa. Nat Cell Biol. 2007; 9(7):797-803. DOI: 10.1038/ncb1605. View

19.

Saint-Criq V, Villeret B, Bastaert F, Kheir S, Hatton A, Cazes A . LasB protease impairs innate immunity in mice and humans by targeting a lung epithelial cystic fibrosis transmembrane regulator-IL-6-antimicrobial-repair pathway. Thorax. 2017; 73(1):49-61. PMC: 5738602. DOI: 10.1136/thoraxjnl-2017-210298. View

20.

Jin Y, Diffee G, Colman R, Anderson R, Ge Y . Top-down Mass Spectrometry of Sarcomeric Protein Post-translational Modifications from Non-human Primate Skeletal Muscle. J Am Soc Mass Spectrom. 2019; 30(12):2460-2469. PMC: 6722035. DOI: 10.1007/s13361-019-02139-0. View