Improvement of Neurological Function in Rats with Ischemic Stroke by Adipose-derived Pericytes

Overview

Journal Cell Transplant

Specialties Cell Biology
General Surgery

Date 2020 Sep 5

PMID 32885682

Citations 5

Authors

Vyacheslav Ogay

Venera Kumasheva

Yelena Li

Sholpan Mukhlis

Aliya Sekenova

Farkhad Olzhayev

Andrey Tsoy

Baurzhan Umbayev

Sholpan Askarova

Azat Shpekov

Assylbek Kaliyev

Berik Zhetpisbayev

Yerbol Makhambetov

Serik Akshulakov

Arman Saparov

Yerlan Ramankulov

Affiliations

Soon will be listed here.

Abstract

Pericytes possess high multipotent features and cell plasticity, and produce angiogenic and neurotrophic factors that indicate their high regenerative potential. The aim of this study was to investigate whether transplantation of adipose-derived pericytes can improve functional recovery and neurovascular plasticity after ischemic stroke in rats. Rat adipose-derived pericytes were isolated from subcutaneous adipose tissue by fluorescence-activated cell sorting. Adult male Wistar rats were subjected to 90 min of middle cerebral artery occlusion followed by intravenous injection of rat adipose-derived pericytes 24 h later. Functional recovery evaluations were performed at 1, 7, 14, and 28 days after injection of rat adipose-derived pericytes. Angiogenesis and neurogenesis were examined in rat brains using immunohistochemistry. It was observed that intravenous injection of adipose-derived pericytes significantly improved recovery of neurological function in rats with stroke compared to phosphate-buffered saline-treated controls. Immunohistochemical analysis revealed that the number of blood capillaries was significantly increased along the ischemic boundary zone of the cortex and striatum in stroke rats treated with adipose-derived pericytes. In addition, treatment with adipose-derived pericytes increased the number of doublecortin positive neuroblasts. Our data suggest that transplantation of adipose-derived pericytes can significantly improve the neurologic status and contribute to neurovascular remodeling in rats after ischemic stroke. These data provide a new insight for future cell therapies that aim to treat ischemic stroke patients.

Citing Articles

Repair of Rat Calvarial Critical-Sized Defects Using Heparin-Conjugated Fibrin Hydrogel Containing BMP-2 and Adipose-Derived Pericytes.

Kudaibergen G, Mukhlis S, Mukhambetova A, Issabekova A, Sekenova A, Sarsenova M Bioengineering (Basel). 2024; 11(5).

PMID: 38790304 PMC: 11117777. DOI: 10.3390/bioengineering11050437.

A new insight into the role of pericytes in ischemic stroke.

Seyedaghamiri F, Geranmayeh M, Ghadiri T, Ebrahimi-Kalan A, Hosseini L Acta Neurol Belg. 2023; 124(3):767-774.

PMID: 37805645 DOI: 10.1007/s13760-023-02391-y.

The therapeutic value of adipose-derived pericyte transplantation after intracerebral hemorrhage in rats.

Zhang X, Zhou Y, Liu Q, Wang F, Fu L, Wei Y J Mol Histol. 2023; 54(5):499-508.

PMID: 37498471 DOI: 10.1007/s10735-023-10140-x.

Regeneration of Osteochondral Defects by Combined Delivery of Synovium-Derived Mesenchymal Stem Cells, TGF-β1 and BMP-4 in Heparin-Conjugated Fibrin Hydrogel.

Sarsenova M, Raimagambetov Y, Issabekova A, Karzhauov M, Kudaibergen G, Akhmetkarimova Z Polymers (Basel). 2022; 14(24).

PMID: 36559710 PMC: 9780905. DOI: 10.3390/polym14245343.

Vascular Biology.

Alkayed N, Cipolla M Stroke. 2021; 52(7):2440-2441.

PMID: 34078110 PMC: 8318183. DOI: 10.1161/STROKEAHA.121.033556.

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