Investigating the Mechanisms of AquaporinZ Reconstitution Through Polymeric Vesicle Composition for a Biomimetic Membrane

Overview

Journal Polymers (Basel)

Publisher MDPI

Date 2020 Sep 3

PMID 32872107

Authors

Danhua Zhou

Hu Zhou

Shufeng Zhou

Yen Wah Tong

Affiliations

Soon will be listed here.

Abstract

Aquaporin-Z (AqpZ) are water channel proteins with excellent water permeability and solute rejection properties. AqpZ can be reconstituted into vesicles utilizing cell-like bilayer membranes assembled from amphiphilic block copolymers, for the preparation of high-performance biomimetic membranes. However, only a few copolymers have been found suitable to act as the membrane matrix for protein reconstitution. Hence, this work analyzes the mechanism of protein reconstitution based on a composition-reconstitution relationship. The vesicle formation and AqpZ reconstitution processes in various amphiphilic block copolymers were investigated in terms of size, morphology, stability, polymeric bilayer membrane rigidity, and thermal behavior. Overall, this study contributes to the understanding of the composition-reconstitution relationship of biomimetic membranes based on AqpZ-reconstituted polymeric vesicles.

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