Formation of PH-Resistant Monodispersed Polymer-Lipid Nanodiscs

Overview

Journal Angew Chem Int Ed Engl

Specialty Chemistry

Date 2017 Dec 13

PMID 29232017

Citations 48

Authors

Thirupathi Ravula

Nathaniel Z Hardin

Sudheer Kumar Ramadugu

Sarah J Cox

Ayyalusamy Ramamoorthy

Affiliations

Soon will be listed here.

Abstract

Polymer lipid nanodiscs are an invaluable system for structural and functional studies of membrane proteins in their near-native environment. Despite the recent advances in the development and usage of polymer lipid nanodisc systems, lack of control over size and poor tolerance to pH and divalent metal ions are major limitations for further applications. A facile modification of a low-molecular-weight styrene maleic acid copolymer is demonstrated to form monodispersed lipid bilayer nanodiscs that show ultra-stability towards divalent metal ion concentration over a pH range of 2.5 to 10. The macro-nanodiscs (>20 nm diameter) show magnetic alignment properties that can be exploited for high-resolution structural studies of membrane proteins and amyloid proteins using solid-state NMR techniques. The new polymer, SMA-QA, nanodisc is a robust membrane mimetic tool that offers significant advantages over currently reported nanodisc systems.

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