Acute Phase Protein Expressions in Secretory and Cistern Lining Epithelium Tissues of the Dairy Cattle Mammary Gland During Chronic Mastitis Caused by Staphylococci

Overview

Journal BMC Vet Res

Publisher Biomed Central

Specialty Veterinary Medicine

Date 2020 Sep 2

PMID 32867772

Citations 2

Authors

M Zalewska

E Kawecka-Grochocka

D Sloniewska

E Kosciuczuk

S Marczak

W Jarmuz

L Zwierzchowski

E Bagnicka

Affiliations

Soon will be listed here.

Abstract

Background: Mastitis is the most common disease in dairy cattle and the costliest for the dairy farming industry, as it lowers milk yield and quality. Mastitis occurs as a result of interactions between microorganisms and the individual genetic predispositions of each animal. Thus, it is important to fully understand the mechanisms underlying these interactions. Elucidating the immune response mechanisms can determine which genetic background makes an animal highly resistant to mastitis. We analyzed the innate immune responses of dairy cows naturally infected with coagulase-positive staphylococci (CoPS; N = 8) or coagulase-negative staphylococci (CoNS; N = 7), causing persistent mastitis (after several failed treatments) vs. infection-free (i.e., healthy [H]; N = 8) dairy cows. The expressions of the acute phase protein genes serum amyloid A3 (SAA3), haptoglobin (HP), ceruloplasmin (CP) genes in the tissues most exposed to pathogens- mammary gland cistern lining epithelial cells (CLECs) and mammary epithelial cells (MECs)-were analyzed.

Results: We found constitutive and extrahepatic expressions of the studied genes in both tissue types. HP expression in the MECs of the CoPS-infected group was higher than in the H group (p ≤ 0.05). Moreover, higher SAA3 expression in the CoPS and CoNS groups than in the H group (p = 0.06 and 0.08, respectively) was found. No differences between SAA3 and HP in CLECs were revealed, regardless of the pathogen type. However, higher expression of CP (p ≤ 0.05) in the CoPS group than in the H group was noted.

Conclusions: The expressions of selected acute phase proteins were similar between CLECs and MECs, which means that CLECs are not only a mechanical barrier but are also responsible for the biological immune response. Our findings agree with the results of other authors describing the immunological response of MECs during chronic mastitis, but the results for CLECs are novel.

Citing Articles

Expression of cytokines in dairy cattle mammary gland parenchyma during chronic staphylococcal infection.

Kawecka-Grochocka E, Zalewska M, Rzewuska M, Kosciuczuk E, Zabek T, Sakowski T Vet Res. 2021; 52(1):132.

PMID: 34663465 PMC: 8522155. DOI: 10.1186/s13567-021-01003-y.

Does Small Ruminant Lentivirus Infection in Goats Predispose to Bacterial Infection of the Mammary Gland? A Preliminary Study.

Urbanska D, Puchala R, Jarczak J, Czopowicz M, Kaba J, Horbanczuk K Animals (Basel). 2021; 11(7).

PMID: 34206282 PMC: 8300097. DOI: 10.3390/ani11071851.

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