Antimicrobial Effectiveness of Calcium Silicate Sealers Against a Nutrient-Stressed Multispecies Biofilm

Overview

Journal J Clin Med

Specialty General Medicine

Date 2020 Aug 28

PMID 32846942

Citations 10

Authors

Rahul Bose

Konstantinos Ioannidis

Federico Foschi

Abdulaziz Bakhsh

Robert D Kelly

Sanjukta Deb

Francesco Mannocci

Sadia Ambreen Niazi

Affiliations

Soon will be listed here.

Abstract

Purpose: This study compared the antimicrobial efficacy of calcium silicate sealers (BioRoot RCS and Total Fill BC) and conventional sealers (AH Plus and Tubli-seal) against planktonic bacteria and a nutrient-stressed multispecies biofilm.

Methods: Antimicrobial properties of freshly mixed sealers were investigated using the direct contact test (DCT) and a nutrient-stressed multispecies biofilm comprised of five endodontic strains. Antimicrobial activity was determined using quantitative viable counts and confocal laser scanning microscopy (CLSM) analysis with live/dead staining. The pH of the sealers was analysed over a period of 28 days in Hanks Balanced Salt Solution (HBSS). Analysis of variance (ANOVA) with Tukey tests and the Kruskal-Wallis test were used for data analysis with a significance of 5%.

Results: All endodontic sealers exhibited significant antimicrobial activity against planktonic bacteria ( < 0.05). BioRoot RCS caused a significant reduction in viable counts of the biofilms compared to AH Plus and the control ( < 0.05), while no significant difference could be observed compared to TotalFill BC and Tubli-seal ( > 0.05). CLSM analysis showed that BioRoot RCS and TotalFill BC exhibited significant biofilm inhibition compared to Tubli-seal, AH Plus and the control ( < 0.05). BioRoot RCS presented with the highest microbial killing, followed by TotalFill BC and Tubli-seal. Alkalizing activity was seen from the onset by BioRoot RCS, TotalFill BC and AH Plus. After 28 days, BioRoot RCS demonstrated the highest pH in HBSS (pH > 12).

Conclusions: Calcium silicate sealers exhibited effective antimicrobial properties. This was demonstrated by superior biofilm inhibition capacity and microbial killing, with strong alkalizing activity compared to epoxy-based and zinc oxide-eugenol-based sealers.

Citing Articles

In Vitro Microscopical and Microbiological Assessment of the Sealing Ability of Calcium Silicate-Based Root Canal Sealers.

Huth K, Wuersching S, Benz L, Kist S, Kollmuss M J Funct Biomater. 2024; 15(11).

PMID: 39590544 PMC: 11595059. DOI: 10.3390/jfb15110341.

Outcomes and prognostic factors of endodontically treated teeth filled with calcium silicate- or epoxy resin-based root canal sealers: A retrospective cohort study.

Kangseng T, Banomyong D, Osiri S, Jantarat J Int Endod J. 2024; 58(1):84-96.

PMID: 39441564 PMC: 11629052. DOI: 10.1111/iej.14155.

Interfacial adaptation of newly prepared nano-tricalcium silicate-58s bioactive glass-based endodontic sealer.

Al-Sabawi N, Al-Jubori S J Dent Res Dent Clin Dent Prospects. 2024; 18(2):115-122.

PMID: 39071217 PMC: 11282200. DOI: 10.34172/joddd.40729.

Biological properties versus solubility of endodontic sealers and cements.

Fristad I, Haug S, Bardsen A Biomater Investig Dent. 2024; 11:40863.

PMID: 38903777 PMC: 11187977. DOI: 10.2340/biid.v11.40863.

Influence of bioceramic sealers on dentinal tubule penetration and antimicrobial effectiveness: a systematic review and meta-analysis of in vitro studies.

Seron M, Nunes G, Ferrisse T, Strazzi-Sahyon H, Dos Santos P, Gomes-Filho J Odontology. 2024; 112(3):672-699.

PMID: 38374511 DOI: 10.1007/s10266-024-00904-6.

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