PGAP6, a GPI-specific Phospholipase A2, Has Narrow Substrate Specificity Against GPI-anchored Proteins

Overview

Journal J Biol Chem

Specialty Biochemistry

Date 2020 Aug 21

PMID 32816994

Citations 10

Authors

Gun-Hee Lee

Morihisa Fujita

Hideki Nakanishi

Haruhiko Miyata

Masahito Ikawa

Yusuke Maeda

Yoshiko Murakami

Taroh Kinoshita

Affiliations

Soon will be listed here.

Abstract

PGAP6, also known as TMEM8A, is a phospholipase A2 with specificity to glycosylphosphatidylinositol (GPI) and expressed on the surface of various cells. CRIPTO, a GPI-anchored co-receptor for a morphogenic factor Nodal, is a sensitive substrate of PGAP6. PGAP6-mediated shedding of CRIPTO plays a critical role in an early stage of embryogenesis. In contrast, CRYPTIC, a close family member of CRIPTO, is resistant to PGAP6. In this report, chimeras between CRIPTO and CRYPTIC and truncate mutants of PGAP6 were used to demonstrate that the Cripto-1/FRL1/Cryptic domain of CRIPTO is recognized by an N-terminal domain of PGAP6 for processing. We also report that among 56 human GPI-anchored proteins tested, only glypican 3, prostasin, SPACA4, and contactin-1, in addition to CRIPTO, are sensitive to PGAP6, indicating that PGAP6 has a narrow specificity toward various GPI-anchored proteins.

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