In Vivo the Antioxidative Extract of Linn. Leaves Induced Apoptosis in Ehrilch Ascites Carcinoma by Modulating P53 Expression

Overview

Journal Food Sci Biotechnol

Specialty Biotechnology

Date 2020 Aug 18

PMID 32802564

Citations 2

Authors

Ayesha Siddika

Tasnim Zahan

Lipy Khatun

Md Rowshanul Habib

Md Abdul Aziz

A R M Tareq

Md Habibur Rahman

Md Rezaul Karim

Affiliations

Soon will be listed here.

Abstract

This study was designed to evaluate the antioxidant activity of methanol extract of Linn. leaves (MELA) using DPPH and ABTS free radical scavenging assays whereas its antineoplastic effect against Ehrlich ascites carcinoma (EAC) was assed using viable cell count, life span, body weight gain and hematological parameters of experimental mice. Results showed that rich phenolic and flavonoid content of MELA had moderate dose dependent free radical scavenging activity (IC: 62.0 μg/mL for DPPH and 6.0 μg/mL for ABTS). In vivo antineoplastic assay, MELA significantly ( < 0.05) decreased viable cells and body weight gain, increased the survival time and restored altered hematological profiles of cancer cell bearing mice. Fluorescence microscopic view of EAC cells derived from MELA-treated group showed apoptotic characteristics and this observation was also supported by overexpression of pro-apoptotic genes coding p53 and Bax proteins in treated cancer cells. The anti-apoptotic genes coding Bcl-2 protein was also absent in treated EAC cells as compared with the control. Moreover, phytochemical profiles of MELA as identified by GC/MS analysis are also consistent with its activities.

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