Comparative Study of the Antioxidant and Anti-Inflammatory Effects of Leaf Extracts from Four Different Genotypes in High Fat Diet-Induced Obesity in Mice

Overview

Journal Antioxidants (Basel)

Date 2020 Aug 16

PMID 32796677

Citations 14

Authors

Francisco Javier Leyva-Jimenez

Antonio Jesus Ruiz-Malagon

Jose Alberto Molina-Tijeras

Patricia Diez-Echave

Teresa Vezza

Laura Hidalgo-Garcia

Jesus Lozano-Sanchez

David Arraez-Roman

Jose Luis Cenis

Antonio Abel Lozano-Perez

Alba Rodriguez-Nogales

Antonio Segura-Carretero

Julio Galvez

Affiliations

Soon will be listed here.

Abstract

Increased levels of reactive oxygen species (ROS) and a low-grade chronic inflammation in multiple organs have been demonstrated in obesity. leaves extracts (MAEs) have been used in traditional medicine as anti-inflammatory agents. In this work, the bioactive compounds of different genotypes of (, , , and ) were analyzed not only by reverse phase high performance liquid chromatography-electrospray ionization-time of flight-mass spectrometry (RP-HPLC-ESI-TOF-MS) and hydrophilic interaction chromatography-electrospray ionization-time of flight-mass spectrometry (HILIC-ESI-TOF-MS), but also screened for in vitro and in vivo antioxidant activity by means of DPPH· radical scavenging assay and model. These MAEs were administered daily in a model of diet-induced obesity in mice. and genotypes significantly reduced weight gain, the glycemic levels in high fat diet, as well as, levels of LDL-cholesterol and triglycerides. and MAEs also reduced the expression of proinflammatory mediators such as , , and increased the levels of adiponectin and AMPK, which exert anti-inflammatory effects. Moreover, genotype ameliorated the intestinal barrier function. In conclusion, and methanolic extracts show the highest antioxidant and anti-inflammatory effect, due to the presence of compounds such as protocatechuic acid or quercetin-3-glucoside, and they could be developed as a complementary treatment for obesity and metabolic disorders.

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