Emergence and Rearrangement of Dynamic Supramolecular Aggregates Visualized by Interferometric Scattering Microscopy

Overview

Journal ACS Nano

Publisher American Chemical Society

Specialty Biotechnology

Date 2020 Aug 14

PMID 32790332

Citations 8

Authors

Maria A Lebedeva

Elena Palmieri

Philipp Kukura

Stephen P Fletcher

Affiliations

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Abstract

Studying dynamic self-assembling systems in their native environment is essential for understanding the mechanisms of self-assembly and thereby exerting full control over these processes. Traditional ensemble-based analysis methods often struggle to reveal critical features of the self-assembly that occur at the single particle level. Here, we describe a label-free single-particle assay to visualize real-time self-assembly in aqueous solutions by interferometric scattering microscopy. We demonstrate how the assay can be applied to biphasic reactions yielding micellar or vesicular aggregates, detecting the onset of aggregate formation, quantifying the kinetics at the single particle level, and distinguishing sigmoidal and exponential growth of aggregate populations. Furthermore, we can follow the evolution in aggregate size in real time, visualizing the nucleation stages of the self-assembly processes and record phenomena such as incorporation of oily components into the micelle or vesicle lumen.

Citing Articles

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From autocatalysis to survival of the fittest in self-reproducing lipid systems.

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Self-reproducing catalytic micelles as nanoscopic protocell precursors.

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Scattering-based Light Microscopy: From Metal Nanoparticles to Single Proteins.

Priest L, Peters J, Kukura P Chem Rev. 2021; 121(19):11937-11970.

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