Dynamic Light Scattering: a Practical Guide and Applications in Biomedical Sciences

Overview

Journal Biophys Rev

Publisher Springer

Specialty Biophysics

Date 2017 May 17

PMID 28510011

Citations 426

Authors

Jorg Stetefeld

Sean A McKenna

Trushar R Patel

Affiliations

Soon will be listed here.

Abstract

Dynamic light scattering (DLS), also known as photon correlation spectroscopy (PCS), is a very powerful tool for studying the diffusion behaviour of macromolecules in solution. The diffusion coefficient, and hence the hydrodynamic radii calculated from it, depends on the size and shape of macromolecules. In this review, we provide evidence of the usefulness of DLS to study the homogeneity of proteins, nucleic acids, and complexes of protein-protein or protein-nucleic acid preparations, as well as to study protein-small molecule interactions. Further, we provide examples of DLS's application both as a complementary method to analytical ultracentrifugation studies and as a screening tool to validate solution scattering models using determined hydrodynamic radii.

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References

Rimai L, Hichmott Jr J, CAREW E, Cole T . Quasi-elastic light scattering by diffusional fluctuations in RNase solutions. Biophys J. 1970; 10(1):20-37. PMC: 1367671. DOI: 10.1016/s0006-3495(70)86283-x. View

Bloomfield V, Lim T . Quasi-elastic laser light scattering. Methods Enzymol. 1978; 48:415-94. DOI: 10.1016/s0076-6879(78)48021-8. View

Lorber B, Fischer F, Bailly M, Roy H, Kern D . Protein analysis by dynamic light scattering: methods and techniques for students. Biochem Mol Biol Educ. 2012; 40(6):372-82. DOI: 10.1002/bmb.20644. View

Pyun J, Cha D, Kwack K . LAMC1 gene is associated with premature ovarian failure. Maturitas. 2012; 71(4):402-6. DOI: 10.1016/j.maturitas.2012.01.011. View

Gerl M, Mann K, Aumailley M, Timpl R . Localization of a major nidogen-binding site to domain III of laminin B2 chain. Eur J Biochem. 1991; 202(1):167-74. DOI: 10.1111/j.1432-1033.1991.tb16358.x. View

Bloomfield V . Quasi-elastic light scattering applications in biochemistry and biology. Annu Rev Biophys Bioeng. 1981; 10:421-50. DOI: 10.1146/annurev.bb.10.060181.002225. View

VAN Holde K . Physical characterization of the protein molecule. Mol Biol Biochem Biophys. 1970; 8:2-24. View

Nitkin R, Smith M, Magill C, Fallon J, Yao Y, Wallace B . Identification of agrin, a synaptic organizing protein from Torpedo electric organ. J Cell Biol. 1987; 105(6 Pt 1):2471-8. PMC: 2114709. DOI: 10.1083/jcb.105.6.2471. View

Nieuwenhuysen P, Clauwaert J . Quasi-elastic light scattering of Artemia ribosomes sedimented in a CsCl density gradient. J Biochem Biophys Methods. 1981; 5(5):279-86. DOI: 10.1016/0165-022x(81)90038-5. View

10.

Hilgenberg L, Su H, Gu H, ODowd D, Smith M . Alpha3Na+/K+-ATPase is a neuronal receptor for agrin. Cell. 2006; 125(2):359-69. DOI: 10.1016/j.cell.2006.01.052. View

11.

Foord R, Jakeman E, Oliver C, Pike E, Blagrove R, Wood E . Determination of diffusion coefficients of haemocyanin at low concentration by intensity fluctuation spectroscopy of scattered laser light. Nature. 1970; 227(5255):242-5. DOI: 10.1038/227242a0. View

12.

Hovanessian A, Justesen J . The human 2'-5'oligoadenylate synthetase family: unique interferon-inducible enzymes catalyzing 2'-5' instead of 3'-5' phosphodiester bond formation. Biochimie. 2007; 89(6-7):779-88. DOI: 10.1016/j.biochi.2007.02.003. View

13.

Mayer U, Nischt R, Poschl E, Mann K, Fukuda K, Gerl M . A single EGF-like motif of laminin is responsible for high affinity nidogen binding. EMBO J. 1993; 12(5):1879-85. PMC: 413408. DOI: 10.1002/j.1460-2075.1993.tb05836.x. View

14.

Deo S, Patel T, Dzananovic E, Booy E, Zeid K, McEleney K . Activation of 2' 5'-oligoadenylate synthetase by stem loops at the 5'-end of the West Nile virus genome. PLoS One. 2014; 9(3):e92545. PMC: 3961380. DOI: 10.1371/journal.pone.0092545. View

15.

Scott D, Patel T, Besong D, Stetefeld J, Winzor D . Examination of the discrepancy between size estimates for ovalbumin from small-angle X-ray scattering and other physicochemical measurements. J Phys Chem B. 2011; 115(36):10725-9. DOI: 10.1021/jp2006149. View

16.

Patel T, Meier M, Li J, Morris G, Rowe A, Stetefeld J . T-shaped arrangement of the recombinant agrin G3-IgG Fc protein. Protein Sci. 2011; 20(6):931-40. PMC: 3104224. DOI: 10.1002/pro.628. View

17.

Hassan P, Kulshreshtha S . Modification to the cumulant analysis of polydispersity in quasielastic light scattering data. J Colloid Interface Sci. 2006; 300(2):744-8. DOI: 10.1016/j.jcis.2006.04.013. View

18.

Fujime S . Quasi-elastic scattering of laser light. A new tool for the dynamic study of biological macromolecules. Adv Biophys. 1972; 3:1-43. View

19.

Harvey J . Diffusion coefficients and hydrodynamic radii of three spherical RNA viruses by laser light scattering. Virology. 1973; 56(1):365-8. DOI: 10.1016/0042-6822(73)90313-9. View

20.

Dzananovic E, Patel T, Deo S, McEleney K, Stetefeld J, McKenna S . Recognition of viral RNA stem-loops by the tandem double-stranded RNA binding domains of PKR. RNA. 2013; 19(3):333-44. PMC: 3677244. DOI: 10.1261/rna.035931.112. View