Quantitative Analysis of β1,6GlcNAc-branched N-glycans on β4 Integrin in Cutaneous Squamous Cell Carcinoma

Overview

Journal Fukushima J Med Sci

Specialty General Medicine

Date 2020 Aug 12

PMID 32779579

Citations 1

Authors

Yoshinobu Kariya

Midori Oyama

Mikio Ohtsuka

Nobuyuki Kikuchi

Yasuhiro Hashimoto

Toshiyuki Yamamoto

Affiliations

Soon will be listed here.

Abstract

α6β4 integrin plays pivotal roles in cancer progression in several types of cancers. Our previous study using N-glycan-manipulated cell lines demonstrated that defects in N-glycans or decreased β1,6GlcNAc-branched N-glycans on β4 integrin suppress β4 integrin-mediated cancer cell adhesion, migration, invasion, and tumorigenesis. Furthermore, immunohistochemical analysis has shown that colocalization of β1,6GlcNAc-branched N-glycans with β4 integrin was observed in cutaneous squamous cell carcinoma (SCC) tissue. However, until now there has been no direct evidence that β1,6GlcNAc-branched N-glycans are upregulated on β4 integrin in cutaneous SCC. In the present study, we performed an ELISA analysis of β1,6GlcNAc-branched N-glycans on β4 integrins as well as β4 integrins in cell lysates from human normal skin and cutaneous SCC tissues. The SCC samples showed a 4.9- to 7.4-fold increase in the ratio of β1,6GlcNAc-branched N-glycans to β4 integrin compared with normal skin samples. These findings suggest that the addition of β1,6GlcNAc-branched N-glycans onto β4 integrin was markedly elevated in cutaneous SCC tissue compared to normal skin tissue. The value of β1,6GlcNAc-branched N-glycans on β4 integrin may be useful as a diagnostic marker associated with cutaneous SCC tumor progression.

Citing Articles

Integrin α6β4 Confers Doxorubicin Resistance in Cancer Cells by Suppressing Caspase-3-Mediated Apoptosis: Involvement of N-Glycans on β4 Integrin Subunit.

Kariya Y, Gu J, Kariya Y Biomolecules. 2023; 13(12).

PMID: 38136623 PMC: 10741852. DOI: 10.3390/biom13121752.

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