Measuring Histamine and Cytokine Release from Basophils and Mast Cells

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2020 Aug 9

PMID 32766982

Citations 3

Authors

Bettina M Jensen

Ewa A Bartko

Katrine Baumann

Per S Skov

Affiliations

Soon will be listed here.

Abstract

Basophils and mast cells are known for their capability to release both preformed and newly synthesized inflammatory mediators. In this chapter, we describe how to stimulate and detect histamine released from basophils in whole blood, purified basophils, in vitro cultured mast cells, and in situ skin mast cells (the latter by microdialysis), using either a solid phase assay or flow cytometry. We also give an example of an activation protocol for basophil and mast cell cytokine release and discuss approaches for cytokine detection.

Citing Articles

Pharmacological blockade of the mast cell MRGPRX2 receptor supports investigation of its relevance in skin disorders.

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