Exploring the LncRNAs Related to Skeletal Muscle Fiber Types and Meat Quality Traits in Pigs

Overview

Journal Genes (Basel)

Publisher MDPI

Date 2020 Aug 8

PMID 32759632

Citations 25

Authors

Rongyang Li

Bojiang Li

Aiwen Jiang

Yan Cao

Liming Hou

Zengkai Zhang

Xiying Zhang

Honglin Liu

Kee-Hong Kim

Wangjun Wu

Affiliations

Soon will be listed here.

Abstract

The alteration in skeletal muscle fiber is a critical factor affecting livestock meat quality traits and human metabolic diseases. Long non-coding RNAs (lncRNAs) are a diverse class of non-coding RNAs with a length of more than 200 nucleotides. However, the mechanisms underlying the regulation of lncRNAs in skeletal muscle fibers remain elusive. To understand the genetic basis of lncRNA-regulated skeletal muscle fiber development, we performed a transcriptome analysis to identify the key lncRNAs affecting skeletal muscle fiber and meat quality traits on a pig model. We generated the lncRNA expression profiles of fast-twitch (Bf) and slow-twitch (Sol) muscles and identified the differentially expressed (DE) lncRNAs using RNA-seq and performed bioinformatics analyses. This allowed us to identify 4581 lncRNA genes among six RNA libraries and 92 DE lncRNAs between Bf and Sol which are the key candidates for the conversion of skeletal muscle fiber types. Moreover, we detected the expression patterns of lncRNA in different tissues and skeletal muscles of various development stages. In addition, we performed a correlation analyses between the expression of DE lncRNA and meat quality traits. Notably, we found that DE lncRNA was highly expressed in skeletal muscle and its expression was significantly higher in Sol than in Bf, with a positive correlation with the expression of () ( = 0.6597, = 0.0016) and a negative correlation with meat quality traits glycolytic potential ( = -0.5447, = 0.0130), as well as drip loss ( = -0.5085, = 0.0221). Moreover, we constructed the lncRNA -mRNAs regulatory network for a better understanding of a possible mechanism regulating skeletal muscle fiber formation. Our data provide the groundwork for studying the lncRNA regulatory mechanisms of skeletal muscle fiber conversion, and given the importance of skeletal muscle fiber types in muscle-related diseases, our data may provide insight into the treatment of muscular diseases in humans.

Citing Articles

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