Enhanced Oral Vaccine Efficacy of Polysaccharide-Coated Calcium Phosphate Nanoparticles

Overview

Journal ACS Omega

Publisher American Chemical Society

Specialty Chemistry

Date 2020 Aug 4

PMID 32743193

Citations 12

Authors

Pei Cao

Felicity Y Han

Lisbeth Grondahl

Zhi Ping Xu

Li Li

Affiliations

Soon will be listed here.

Abstract

Oral administration of vaccines has been limited due to low immune response compared to parenteral administration. Antigen degradation in the acidic gastrointestinal environment (GI), mucus barriers, and inefficient cellular uptake by immune cells are the major challenges for oral vaccine delivery. To solve these issues, the current study investigates calcium phosphate nanoparticles (CaP NPs) coated with polysaccharides as nanocarriers for oral protein antigen delivery. In this design, the CaP NP core had an optimized antigen encapsulation capacity of 90 mg (BSA-FITC)/g (CaP NPs). The polysaccharides chitosan and alginate were coated onto the CaP NPs to protect the antigens against acidic degradation in the GI environment and enhance the immune response in the small intestine. The antigen release profiles showed that alginate-chitosan-coated CaP NPs prevented antigen release in a simulated gastric fluid (pH 1.2), followed by sustained release in simulated intestinal (pH 6.8) and colonic (pH 7.4) fluids. Cellular uptake and macrophage stimulation data revealed that the chitosan coating enhanced antigen uptake by intestine epithelia cells (Caco-2) and macrophages and improved surface expression of costimulatory molecules on macrophages. test further demonstrated that oral administration of alginate-chitosan-coated CaP@OVA NPs significantly enhanced the mucosal IgA and serum IgG antibody responses as compared to naked OVA, indicating that the CaP-Chi-Alg nanoparticle can potentially be used as a promising oral vaccine delivery system.

Citing Articles

Amelioration of LPS-Induced Jejunum Injury and Mucus Barrier Damage in Mice by IgY Embedded in W/O/W Emulsion.

Wang Z, Ye R, Zhang S, Liu C, Chen K, Zhu K Foods. 2025; 13(24.

PMID: 39767078 PMC: 11675984. DOI: 10.3390/foods13244138.

Recent advances in essential oils and their nanoformulations for poultry feed.

Movahedi F, Nirmal N, Wang P, Jin H, Grondahl L, Li L J Anim Sci Biotechnol. 2024; 15(1):110.

PMID: 39123220 PMC: 11316336. DOI: 10.1186/s40104-024-01067-8.

Chitosan non-particulate vaccine delivery systems.

Masimov R, Wasan E J Pharm Pharm Sci. 2024; 27:12921.

PMID: 39114808 PMC: 11303186. DOI: 10.3389/jpps.2024.12921.

Development of polysaccharide-coated layered double hydroxide nanocomposites for enhanced oral insulin delivery.

Pang H, Wu Y, Chen Y, Chen C, Nie X, Li P Drug Deliv Transl Res. 2024; 14(9):2345-2355.

PMID: 38214820 PMC: 11291568. DOI: 10.1007/s13346-023-01504-7.

Advances in Oral Drug Delivery Systems: Challenges and Opportunities.

Lou J, Duan H, Qin Q, Teng Z, Gan F, Zhou X Pharmaceutics. 2023; 15(2).

PMID: 36839807 PMC: 9960885. DOI: 10.3390/pharmaceutics15020484.

References

Cole H, Bryan D, Lancaster L, Mawas F, Vllasaliu D . Chitosan nanoparticle antigen uptake in epithelial monolayers can predict mucosal but not systemic in vivo immune response by oral delivery. Carbohydr Polym. 2018; 190:248-254. DOI: 10.1016/j.carbpol.2018.02.084. View

Qi J, Zhuang J, Lv Y, Lu Y, Wu W . Exploiting or overcoming the dome trap for enhanced oral immunization and drug delivery. J Control Release. 2018; 275:92-106. DOI: 10.1016/j.jconrel.2018.02.021. View

Muheem A, Shakeel F, Jahangir M, Anwar M, Mallick N, Kumar Jain G . A review on the strategies for oral delivery of proteins and peptides and their clinical perspectives. Saudi Pharm J. 2016; 24(4):413-28. PMC: 4908063. DOI: 10.1016/j.jsps.2014.06.004. View

Yun Y, Cho Y, Park K . Nanoparticles for oral delivery: targeted nanoparticles with peptidic ligands for oral protein delivery. Adv Drug Deliv Rev. 2012; 65(6):822-32. PMC: 3574626. DOI: 10.1016/j.addr.2012.10.007. View

Wu Y, Gu W, Li L, Chen C, Xu Z . Enhancing PD-1 Gene Silence in T Lymphocytes by Comparing the Delivery Performance of Two Inorganic Nanoparticle Platforms. Nanomaterials (Basel). 2019; 9(2). PMC: 6410115. DOI: 10.3390/nano9020159. View

Uskokovic V, Uskokovic D . Nanosized hydroxyapatite and other calcium phosphates: chemistry of formation and application as drug and gene delivery agents. J Biomed Mater Res B Appl Biomater. 2010; 96(1):152-91. DOI: 10.1002/jbm.b.31746. View

Saringer S, Akula R, Szerlauth A, Szilagyi I . Papain Adsorption on Latex Particles: Charging, Aggregation, and Enzymatic Activity. J Phys Chem B. 2019; 123(46):9984-9991. DOI: 10.1021/acs.jpcb.9b08799. View

Tang J, Li L, Howard C, Mahler S, Huang L, Xu Z . Preparation of optimized lipid-coated calcium phosphate nanoparticles for enhanced in vitro gene delivery to breast cancer cells. J Mater Chem B. 2016; 3(33):6805-6812. PMC: 4869335. DOI: 10.1039/C5TB00912J. View

Serag M, Kaji N, Gaillard C, Okamoto Y, Terasaka K, Jabasini M . Trafficking and subcellular localization of multiwalled carbon nanotubes in plant cells. ACS Nano. 2010; 5(1):493-9. DOI: 10.1021/nn102344t. View

10.

Morishita M, Peppas N . Is the oral route possible for peptide and protein drug delivery?. Drug Discov Today. 2006; 11(19-20):905-10. DOI: 10.1016/j.drudis.2006.08.005. View

11.

Jani P, Halbert G, Langridge J, Florence A . Nanoparticle uptake by the rat gastrointestinal mucosa: quantitation and particle size dependency. J Pharm Pharmacol. 1990; 42(12):821-6. DOI: 10.1111/j.2042-7158.1990.tb07033.x. View

12.

Yu X, Wen T, Cao P, Shan L, Li L . Alginate-chitosan coated layered double hydroxide nanocomposites for enhanced oral vaccine delivery. J Colloid Interface Sci. 2019; 556:258-265. DOI: 10.1016/j.jcis.2019.08.027. View

13.

Lin Y, Wang X, Huang X, Zhang J, Xia N, Zhao Q . Calcium phosphate nanoparticles as a new generation vaccine adjuvant. Expert Rev Vaccines. 2017; 16(9):895-906. DOI: 10.1080/14760584.2017.1355733. View

14.

Han Y, Zhao L, Yu Z, Feng J, Yu Q . Role of mannose receptor in oligochitosan-mediated stimulation of macrophage function. Int Immunopharmacol. 2005; 5(10):1533-42. DOI: 10.1016/j.intimp.2005.04.015. View

15.

des Rieux A, Fievez V, Garinot M, Schneider Y, Preat V . Nanoparticles as potential oral delivery systems of proteins and vaccines: a mechanistic approach. J Control Release. 2006; 116(1):1-27. DOI: 10.1016/j.jconrel.2006.08.013. View

16.

Snook J, Chesson C, Peniche A, Dann S, Paulucci A, Pinchuk I . Peptide nanofiber-CaCO composite microparticles as adjuvant-free oral vaccine delivery vehicles. J Mater Chem B. 2020; 4(9):1640-1649. DOI: 10.1039/c5tb01623a. View

17.

Ghaffarian R, Herrero E, Oh H, Raghavan S, Muro S . Chitosan-Alginate Microcapsules Provide Gastric Protection and Intestinal Release of ICAM-1-Targeting Nanocarriers, Enabling GI Targeting In Vivo. Adv Funct Mater. 2016; 26(20):3382-3393. PMC: 4926773. DOI: 10.1002/adfm.201600084. View

18.

Pavlovic M, Rouster P, Szilagyi I . Synthesis and formulation of functional bionanomaterials with superoxide dismutase activity. Nanoscale. 2016; 9(1):369-379. DOI: 10.1039/c6nr07672f. View

19.

Truong-Le V, Lovalenti P, Abdul-Fattah A . Stabilization challenges and formulation strategies associated with oral biologic drug delivery systems. Adv Drug Deliv Rev. 2015; 93:95-108. DOI: 10.1016/j.addr.2015.08.001. View

20.

Sokolova V, Knuschke T, Kovtun A, Buer J, Epple M, Westendorf A . The use of calcium phosphate nanoparticles encapsulating Toll-like receptor ligands and the antigen hemagglutinin to induce dendritic cell maturation and T cell activation. Biomaterials. 2010; 31(21):5627-33. DOI: 10.1016/j.biomaterials.2010.03.067. View