In Vivo Imaging of the Hyaloid Vascular Regression and Retinal and Choroidal Vascular Development in Rat Eyes Using Optical Coherence Tomography Angiography

Overview

Journal Sci Rep

Specialty Science

Date 2020 Aug 1

PMID 32733052

Citations 2

Authors

YongJoo Kim

Jang Ryul Park

Hye Kyoung Hong

Myounghee Han

Jingu Lee

Pilhan Kim

Se Joon Woo

Kyu Hyung Park

Wang-Yuhl Oh

Affiliations

Soon will be listed here.

Abstract

This study investigates the hyaloid vascular regression and its relationship to the retinal and choroidal vascular developments using optical coherence tomography angiography (OCTA). Normal and oxygen-induced retinopathy (OIR) rat eyes at postnatal day 15, 18, 21, and 24 were longitudinally imaged using OCTA. At each day, two consecutive imaging for visualizing the hyaloid vasculature and the retinal and choroidal vasculatures were conducted. The hyaloid vessel volume and the retinal and choroidal vessel densities were measured. The hyaloid vessel volumes gradually decreased during the regression, although the OIR eyes exhibited large vessel volumes at all time points. A spatial relationship between persistent hyaloid vasculature and retardation of underlying retinal vascular development was observed in the OIR eyes. Furthermore, anti-vascular endothelial growth factor (VEGF) was administered intravitreally to additional OIR eyes to observe its effect on the vascular regression and development. The VEGF injection to OIR eyes showed reduced persistent hyaloid vessels in the injected eyes as well as in the non-injected fellow eyes. This study presents longitudinal imaging of intraocular vasculatures in the developing eye and shows the utility of OCTA that can be widely used in studies of vascular development and regression and preclinical evaluation of new anti-angiogenic drugs.

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