Alteration of Immunophenotype of Human Macrophages and Monocytes After Exposure to Cigarette Smoke

Overview

Journal Sci Rep

Specialty Science

Date 2020 Aug 1

PMID 32732964

Citations 19

Authors

Camila Oliveira da Silva

Thomas Gicquel

Yoann Daniel

Thiago Bartholo

Elise Vene

Pascal Loyer

Luis Cristovao Porto

Vincent Lagente

Tatiana Victoni

Affiliations

Soon will be listed here.

Abstract

Cigarette smoke exposure (CS) is the main risk factor for chronic obstructive pulmonary disease (COPD). Macrophages have an important role in COPD because they release pro-inflammatory and anti-inflammatory cytokines. The present study's we investigate the functional changes in macrophages and monocytes exposed to cigarette smoke extract (CSE). Herein, using human monocyte-derived macrophages (MDMs) from healthy donors and we found that CSE was not associated with significant changes in the production of pro inflammatory cytokines by MDMs. In contrast, exposure to CSE suppressed the production of IL-6 and Gro-a/CXCL1 by LPS-stimulated-MDMs, but had an additive effect on the release of IL-8/CXCL8 and MCP1/CCL2. However, CSE exposure was associated with greater production, TARC/CCL-17 and CCL22/MDC. Moreover, MDMs displayed a lower uptake capacity after CSE exposure. We identify, for what is to our knowledge the first time that monocytes from patients with COPD produced less IL-8/CXCL8 and Gro-α/CXCL1 after LPS stimulation and produced higher levels of TARC/CCL17 and MDC/CCL-22 after IL-4 stimulation. Our present results highlighted a skewed immune response, with an imbalance in M1 vs. M2 cytokine production. In conclusion, exposure to CS has contrasting, multifaceted effects on macrophages and monocytes. Our data may provide a better understanding of the mechanisms underlying COPD.

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References

Mantovani A, Biswas S, Galdiero M, Sica A, Locati M . Macrophage plasticity and polarization in tissue repair and remodelling. J Pathol. 2012; 229(2):176-85. DOI: 10.1002/path.4133. View

Almeida J, Bueno C, Alguero M, Sanchez M, de Santiago M, Escribano L . Comparative analysis of the morphological, cytochemical, immunophenotypical, and functional characteristics of normal human peripheral blood lineage(-)/CD16(+)/HLA-DR(+)/CD14(-/lo) cells, CD14(+) monocytes, and CD16(-) dendritic cells. Clin Immunol. 2001; 100(3):325-38. DOI: 10.1006/clim.2001.5072. View

Buhl R, Bals R, Baur X, Berdel D, Criee C, Gappa M . [Guideline for the Diagnosis and Treatment of Asthma - Guideline of the German Respiratory Society and the German Atemwegsliga in Cooperation with the Paediatric Respiratory Society and the Austrian Society of Pneumology]. Pneumologie. 2018; 71(12):e3. DOI: 10.1055/a-0790-0021. View

Dey A, Allen J, Hankey-Giblin P . Ontogeny and polarization of macrophages in inflammation: blood monocytes versus tissue macrophages. Front Immunol. 2015; 5:683. PMC: 4303141. DOI: 10.3389/fimmu.2014.00683. View

Bazzan E, Turato G, Tine M, Radu C, Balestro E, Rigobello C . Dual polarization of human alveolar macrophages progressively increases with smoking and COPD severity. Respir Res. 2017; 18(1):40. PMC: 5324331. DOI: 10.1186/s12931-017-0522-0. View

Oliveira da Silva C, Monte-Alto-Costa A, Renovato-Martins M, Viana Nascimento F, Valenca S, Lagente V . Time Course of the Phenotype of Blood and Bone Marrow Monocytes and Macrophages in the Lung after Cigarette Smoke Exposure In Vivo. Int J Mol Sci. 2017; 18(9). PMC: 5618589. DOI: 10.3390/ijms18091940. View

Hodge S, Hodge G, Scicchitano R, Reynolds P, Holmes M . Alveolar macrophages from subjects with chronic obstructive pulmonary disease are deficient in their ability to phagocytose apoptotic airway epithelial cells. Immunol Cell Biol. 2003; 81(4):289-96. DOI: 10.1046/j.1440-1711.2003.t01-1-01170.x. View

Ziegler-Heitbrock L . The CD14+ CD16+ blood monocytes: their role in infection and inflammation. J Leukoc Biol. 2006; 81(3):584-92. DOI: 10.1189/jlb.0806510. View

Traves S, Smith S, Barnes P, Donnelly L . Specific CXC but not CC chemokines cause elevated monocyte migration in COPD: a role for CXCR2. J Leukoc Biol. 2004; 76(2):441-50. DOI: 10.1189/jlb.1003495. View

10.

Ritter M, Goggel R, Chaudhary N, Wiedenmann A, Jung B, Weith A . Elevated expression of TARC (CCL17) and MDC (CCL22) in models of cigarette smoke-induced pulmonary inflammation. Biochem Biophys Res Commun. 2005; 334(1):254-62. DOI: 10.1016/j.bbrc.2005.06.084. View

11.

Donnelly L, Barnes P . Defective phagocytosis in airways disease. Chest. 2012; 141(4):1055-1062. DOI: 10.1378/chest.11-2348. View

12.

Eapen M, Hansbro P, McAlinden K, Kim R, Ward C, Hackett T . Abnormal M1/M2 macrophage phenotype profiles in the small airway wall and lumen in smokers and chronic obstructive pulmonary disease (COPD). Sci Rep. 2017; 7(1):13392. PMC: 5645352. DOI: 10.1038/s41598-017-13888-x. View

13.

Ziegler-Heitbrock L . Blood Monocytes and Their Subsets: Established Features and Open Questions. Front Immunol. 2015; 6:423. PMC: 4538304. DOI: 10.3389/fimmu.2015.00423. View

14.

Dragoljevic D, Kraakman M, Nagareddy P, Ngo D, Shihata W, Kammoun H . Defective cholesterol metabolism in haematopoietic stem cells promotes monocyte-driven atherosclerosis in rheumatoid arthritis. Eur Heart J. 2018; 39(23):2158-2167. PMC: 6001889. DOI: 10.1093/eurheartj/ehy119. View

15.

Vene E, Barouti G, Jarnouen K, Gicquel T, Rauch C, Ribault C . Opsonisation of nanoparticles prepared from poly(β-hydroxybutyrate) and poly(trimethylene carbonate)-b-poly(malic acid) amphiphilic diblock copolymers: Impact on the in vitro cell uptake by primary human macrophages and HepaRG hepatoma cells. Int J Pharm. 2016; 513(1-2):438-452. DOI: 10.1016/j.ijpharm.2016.09.048. View

16.

Wang Y, Luo G, Chen J, Jiang R, Zhu J, Hu N . Cigarette smoke attenuates phagocytic ability of macrophages through down-regulating Milk fat globule-EGF factor 8 (MFG-E8) expressions. Sci Rep. 2017; 7:42642. PMC: 5307389. DOI: 10.1038/srep42642. View

17.

Winkler A, Nocka K, Sulahian T, Kobzik L, Williams C . In vitro modeling of human alveolar macrophage smoke exposure: enhanced inflammation and impaired function. Exp Lung Res. 2008; 34(9):599-629. DOI: 10.1080/01902140802366261. View

18.

Cornwell W, Kim V, Fan X, Vega M, Ramsey F, Criner G . Activation and polarization of circulating monocytes in severe chronic obstructive pulmonary disease. BMC Pulm Med. 2018; 18(1):101. PMC: 6003040. DOI: 10.1186/s12890-018-0664-y. View

19.

Chen H, Cowan M, Hasday J, Vogel S, Medvedev A . Tobacco smoking inhibits expression of proinflammatory cytokines and activation of IL-1R-associated kinase, p38, and NF-kappaB in alveolar macrophages stimulated with TLR2 and TLR4 agonists. J Immunol. 2007; 179(9):6097-106. DOI: 10.4049/jimmunol.179.9.6097. View

20.

Barnes P . Inflammatory mechanisms in patients with chronic obstructive pulmonary disease. J Allergy Clin Immunol. 2016; 138(1):16-27. DOI: 10.1016/j.jaci.2016.05.011. View